Dkk-1, Human, Quantikine ELISA Kit
掲載日情報：2018/02/01 現在Webページ番号：206219
R&D Systems製のDkk-1, Human,を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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Dkk-1, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
Dkk-1, Human, ELISA Kit, Quantikine (96well)
文献数： 15
- 商品コード：DKK100B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：カルタヘナ該当品
| 説明文 |
感度：0.948 pg/mL，測定範囲：9.38 - 600 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum-free Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Sandwich，アッセイ数：96，別売品コントロール：QC241，測定時間：4.5 hours，測定因子：Dkk-1 別名：dickkopf (Xenopus laevis) homolog 1 Genbank No： 22943 |
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| 法規制等 | カルタヘナ該当品 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 uL), Serum-free Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL)
- Sensitivity
- 0.948 pg/mL
- Assay Range
- 9.4 - 600 pg/mL (Cell Culture Supernates, Serum-free Cell Culture Supern, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant human Dkk-1.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine® Human Dkk-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure human Dkk-1 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human Dkk-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Dkk-1 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Dkk-1.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum-free Cell Culture Supern, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
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Recovery
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 91 | 83-101 |
| Platelet-poor EDTA Plasma (n=4) | 95 | 91-98 |
| Platelet-poor Heparin Plasma (n=4) | 99 | 93-105 |
| Serum-free Cell Culture Media (n=4) | 82 | 77-90 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Dkk-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay as directed in the Sample Preparation section.
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Additional Infomation
- Entrez Gene IDs
- 22943 (Human); 13380 (Mouse)
- Background
- Dkk-1
- Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.
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Citations
Authors: EJ Lee, SM Kim, B Choi, EY Kim, YH Chung, EJ Lee, B Yoo, CK Lee, S Hong, BJ Kim, JM Koh, SH Kim, YG Kim, EJ Chang
Sci Rep, 2017;7(0):40240.
Species: Human
Sample Type: Plasma
Authors: CC Niu, SS Lin, LJ Yuan, LH Chen, CY Yang, AN Chung, ML Lu, TT Tsai, PL Lai, WJ Chen
BMC Musculoskelet Disord, 2017;18(1):61.
Species: Human
Sample Type: Serum
Authors: TH Kim, JS Chang, KS Park, J Park, N Kim, JI Lee, ID Kong
PLoS ONE, 2017;12(2):e0171771.
Species: Human
Sample Type: Serum
Authors: X Tang, J Lin, G Wang, J Lu
PLoS ONE, 2017;12(6):e0179860.
Species: Rat
Sample Type: Serum
Authors: DR Jadon, R Sengupta, A Nightingal, H Lu, J Dunphy, A Green, JT Elder, RP Nair, E Korendowyc, MA Lindsay, NJ McHugh
Arthritis Res. Ther., 2017;19(1):210.
Species: Human
Sample Type: Serum
Authors: Hartman M, Talar B, Noman M, Gajos-Michniewicz A, Chouaib S, Czyz M
PLoS ONE, 2014;9(4):e95157.
Species: Human
Sample Type: Cell Culture Supernates
Authors: Andre T, Meuleman N, Stamatopoulos B, De Bruyn C, Pieters K, Bron D, Lagneaux L
PLoS ONE, 2013;8(3):e59756.
Species: Human
Sample Type: Cell Culture Supernates
Authors: Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R
Cell, 2011;145(6):926-40.
Authors: Gunn WG, Krause U, Lee N, Gregory CA
Blood, 2011;117(5):1641-51.
Species: Mouse
Sample Type: Serum
Authors: Honsawek S, Tanavalee A, Yuktanandana P, Ngarmukos S, Saetan N, Tantavisut S
BMC Musculoskelet Disord, 2010;11(0):257.
Species: Human
Sample Type: Plasma
Authors: Olivares-Navarrete R, Hyzy S, Wieland M, Boyan BD, Schwartz Z
Biomaterials, 2010;31(8):2015-24.
Species: Human
Sample Type: Cell Culture Supernates
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