Human NT-ProANP Quantikine ELISA Kit
掲載日情報:2018/02/01 現在Webページ番号:206210
R&D Systems製のHuman NT-ProANPを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 :2025年04月28日 16時55分現在]
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[在庫・価格 :2025年04月28日 16時55分現在]
Human NT-ProANP Quantikine ELISA Kit
文献数: 1
- 商品コード:DANP00
- メーカー:RSD
- 包装:1kit
- 価格:¥116,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 感度:0.146 ng/mL,測定範囲:0.313-20 ng/mL,測定試料:Serum, Plasma, Cell Culture Supernatant,種交差性:Human,検出方法:colorimetric,測定波長:450 nm,アッセイ法:Sandwich,アッセイ数:96,別売品コントロール:QC227,測定時間:4.5 hours,測定因子:NT-Pro Atrial Natriuretic Peptide, ANP 別名:ANF Genbank No: 4878 |
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保存条件 | 4℃ | 法規備考 | |
掲載カタログ |
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製品記事 | Quantikine Human NT-ProANP ELISA Kit ELISA Kit製品に関するFAQ(R&D Systems) |
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関連記事 | R&D Systems Quantikine ELISA Kit/DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
- Sensitivity
- 0.146 ng/mL
- Assay Range
- 0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
- Specificity
- Natural and recombinant human NT-ProANP.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Recovery
The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
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Cell Culture Media (n=4) | 104 | 87-114 |
EDTA Plasma (n=4) | 102 | 84-120 |
Heparin Plasma (n=4) | 98 | 84-111 |
Serum (n=4) | 108 | 95-122 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human NT-ProANP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Entrez Gene IDs
- 4878 (Human); 230899 (Mouse); 24602 (Rat)
- Background
- Atrial Natriuretic Peptide/ANP
- Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.
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