抗Mouse SLAM/CD150抗体(Anti-Mouse SLAM/CD150 antibody)
掲載日情報：2021/01/28 現在Webページ番号：199458
Mouse SLAM/CD150に対する抗体(Anti-Mouse SLAM/CD150 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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Anti-Mouse SLAM/CD150 MAb (Clone 459911) (100 UG) |
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Anti-Mouse SLAM/CD150 MAb (Clone 459911) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-Mouse SLAM/CD150 MAb (Clone 459911) (100 UG)
文献数： 3
- 商品コード：MAB4330
- メーカー：RSD
- 包装：100μg
- 価格：¥91,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse SLAM/CD150 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF4330，スタンダードとして#4330-SL-050を用いる。 別名：CD 150 クローン：459911 Genbank No： 6504 Protein Accession No： Q9QUM4 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Rat | ||||
| 交差性 | Mouse | 適用 | ELISA,FCM | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse SLAM/CD150 MAb (Clone 459911)
文献数： 0
- 商品コード：MAB4330-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：CD 150 クローン：459911 Genbank No： 6504 Protein Accession No： Q9QUM4 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,FCM | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150Thr25-Pro242Accession # Q9QUM4 |
| Source | Monoclonal Rat IgG2A Clone # 459911 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse SLAM/CD150. |
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Applications and Data
| Recommended Concentration | Sample | |
| Flow Cytometry | 2.5 µg/106 cells | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Mouse SLAM/CD150 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse SLAM/CD150 Antibody (Catalog #MAB4330 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse SLAM/CD150 Biotinylated Antibody (Catalog #BAF4330 ) |
| ELISA Standard | Recombinant Mouse SLAM/CD150 Protein, CF (Catalog #4330-SL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Flow Cytometry |
 click image to view larger | Detection of SLAM/CD150 in Mouse Splenocytes by Flow Cytometry.Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin then stained with Mouse SLAM/CD150 Monoclonal Antibody (Catalog # MAB4330, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: SLAM/CD150 | The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4+ and CD8+ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8+ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14). |
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References
| Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337. | |
| Castro, A.G. et al. (1999) J. Immunol. 163:5860. | |
| Cocks, B.G. et al. (1995) Nature 376:260. | |
| Wang, N. et al. (2004) J. Exp. Med. 199:1255. | |
| Hahm, B. et al. (2004) Virology 323:292. | |
| Nanda, N. et al. (2005) Blood 106:3028. | |
| Kiel, M.J. et al. (2005) Cell 121:1109. | |
| Punnonen, J. et al. (1997) J. Exp. Med. 185:993. | |
| Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100. | |
| Aversa, G. et al. (1997) J. Immunol. 158:4036. | |
| Mehrle, S. et al. (2008) Mol. Immunol. 45:796. | |
| Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206. | |
| Tatsuo, H. et al. (2000) Nature 406:893. | |
| Hsu, E.C. et al. (2001) Virology 279:9. | |
| Long Name: | Signaling Lymphocytic Activation Molecule |
| Entrez Gene IDs: | 6504 (Human); 27218 (Mouse) |
| Alternate Names: | CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1 |
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Related Research Areas
| B Cell Activation | ||
| Co-stimulatory and Co-inhibitory Molecules | ||
| Co-stimulatory Molecules Associated with Tfh Cells | ||
| Dendritic Cell Co-stimulatory Molecules | ||
| Dendritic Cell ITIM/ITAM Immunoreceptors | ||
| Endothelial Cell Markers | ||
| Hematopoietic Stem Cell Markers | ||
| ITIM/ITAM Immunoreceptors and Related Molecules | ||
| Lymphoma | ||
| Macrophage Activation Markers | ||
| Megakaryocytes | ||
| Monocyte Markers | ||
| Natural Killer T (NKT) Cells | ||
| Platelet Immune Receptors | ||
| Regulatory T Cells (Tregs) | ||
| ||
| Flow Cytometry | |
|---|---|
 | Detection of SLAM/CD150 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin then stained with Mouse SLAM/CD150 Monoclonal Antibody (Catalog # MAB4330, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). |
追加しました。
Related Product & Information
| Background | SLAM/CD150 |
|---|---|
| background_content | Background: SLAM/CD150 The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4+ and CD8+ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8+ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14). |
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Citations
- Identification of a clonally expanding haematopoietic compartment in bone marrow.
Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R
EMBO J, 2013;32(2):219-30.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Fluorescence
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