抗Mouse Erythropoietin Biotinylated抗体(Anti-Mouse Erythropoietin Biotinylated antibody)
掲載日情報:2021/01/28 現在Webページ番号:195237
Mouse Erythropoietin Biotinylatedに対する抗体(Anti-Mouse Erythropoietin Biotinylated )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
価格
[在庫・価格 :2024年05月20日 16時35分現在]
詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Anti-Mouse Erythropoietin Biotinylated MAb |
|
0 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
[在庫・価格 :2024年05月20日 16時35分現在]
Anti-Mouse Erythropoietin Biotinylated MAb
文献数: 0
- 商品コード:BAM9592
- メーカー:RSD
- 包装:250μg
- 価格:¥118,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Mouse Erythropoietin サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB9591-500,スタンダードとして#959-ME-010を用いる。 別名:EP クローン:148409 Genbank No: 2056 Protein Accession No: Q0VED9 |
||
---|---|---|---|
法規制等 | |||
保存条件 | -20℃ | 法規備考 | |
抗原種 | 免疫動物 | Rat | |
交差性 | Mouse | 適用 | ELISA |
標識 | Biotin | 性状 | Protein A/G Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Monoclonal | フォーマット | |
掲載カタログ |
|
||
製品記事 |
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
||
関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
追加しました。
Product Details
Species Reactivity | Mouse |
---|---|
Label | Biotin |
Immunogen | Chinese hamster ovary cell line CHO-derived recombinant mouse ErythropoietinAla27-Arg192Accession # Q0VED9 |
Source | Monoclonal Rat IgG2B Clone # 148409 |
Purification | Antigen and protein G Affinity-purified from hybridoma culture supernatant |
Specificity | Detects mouse Erythropoietin in ELISAs. In sandwich immunoassays, 100% cross-reactivity with canine, human and rat Erythropoietin is observed and no cross-reactivity with recombinant mouse Thrombopoietin is observed. |
追加しました。
Applications and Data
Recommended Concentration | Sample | |
Mouse Erythropoietin Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse Erythropoietin Antibody (Catalog #MAB9591 ) |
ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Mouse Erythropoietin Biotinylated Antibody (Catalog #BAM9592 ) |
ELISA Standard | Recombinant Mouse Erythropoietin Protein (Catalog #959-ME ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: Erythropoietin | Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N‑glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of Epo is required for biological activities in vivo (4). Mature mouse Epo shares 95% amino acid sequence identity with rat Epo and 73%‑82% with bovine, canine, equine, feline, human, ovine, and porcine Epo. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6‑8). Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R) (8, 9). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). Epo additionally plays a tissue‑protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13). |
追加しました。
References
Koury, M. J. (2005) Exp. Hematol. 33:1263. | |
Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849. | |
Wen, D. et al. (1993) Blood 82:1507. | |
Tsuda E. et al. (1990) Eur. J. Biochem. 188:405. | |
Lacombe, C. et al. (1988) J. Clin. Invest. 81:620. | |
Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13. | |
Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184. | |
Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025. | |
Koury, M.J. and M.C. Bondurant (1990) Science 248:378. | |
Acs, G. et al. (2001) Cancer Res. 61:3561. | |
Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332. | |
Verdier, F. et al. (2000) J. Biol. Chem. 275:18375. | |
Kertesz, N. et al. (2004) Dev. Biol. 276:101. | |
Entrez Gene IDs: | 2056 (Human); 13856 (Mouse); 24335 (Rat) |
Alternate Names: | EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 |
追加しました。
Related Research Areas
Cytokine and Growth Factor Receptors on VSMC | ||||||||||
HIF Transcription Factors | ||||||||||
Red Blood Cells (RBCs) | ||||||||||
Renal Markers | ||||||||||
Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
---|---|---|---|---|---|---|---|---|---|---|
Enlarge | Very Good | ELISA | Canine | Anonymous | 12/06/2017 | Image Details |
ELISA: Mouse Erythropoietin Biotinylated Antibody [BAM9592]. |
追加しました。
Summary
Application | ELISA |
Sample Tested | Recombinant protein |
Species | Canine |
追加しました。
Related Product & Information
Background | Erythropoietin |
---|---|
background_content | Background: Erythropoietin Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N‑glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of Epo is required for biological activities in vivo (4). Mature mouse Epo shares 95% amino acid sequence identity with rat Epo and 73%‑82% with bovine, canine, equine, feline, human, ovine, and porcine Epo. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6‑8). Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R) (8, 9). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). Epo additionally plays a tissue‑protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13). |
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。