抗Human G-CSF抗体(Anti-Human G-CSF antibody)
掲載日情報：2021/01/28 現在Webページ番号：179195
Human G-CSFに対する抗体(Anti-Human G-CSF )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年05月01日 00時00分現在]
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Anti-Human G-CSF MAb (Clone 3316R) |
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Anti-Human G-CSF MAb (Clone 3316R) |
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[在庫・価格 ：2025年05月01日 00時00分現在]
Anti-Human G-CSF MAb (Clone 3316R)
文献数： 0
- 商品コード：MAB214R-01M
- メーカー：RSD
- 包装：1mg
- 価格：ご照会ください
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：C17orf33 クローン：3316R Genbank No： 1440 Protein Accession No： NP_757373 |
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,Neutralising |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human G-CSF MAb (Clone 3316R)
文献数： 0
- 商品コード：MAB214R-100
- メーカー：RSD
- 包装：100μg
- 価格：¥103,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human G-CSF サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF214，スタンダードとして#214-CS-005を用いる。 別名：C17orf33 クローン：3316R Genbank No： 1440 Protein Accession No： NP_757373 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Recombinant | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human G-CSF MAb (Clone 3316R)
文献数： 0
- 商品コード：MAB214R-500
- メーカー：RSD
- 包装：500μg
- 価格：¥245,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：C17orf33 クローン：3316R Genbank No： 1440 Protein Accession No： NP_757373 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,Neutralising |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
Anti-Human G-CSF MAb (Clone 3316R)
文献数： 0
- 商品コード：MAB214R-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。 別名：C17orf33 クローン：3316R Genbank No： 1440 Protein Accession No： NP_757373 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Recombinant | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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追加しました。
Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human G-CSFAla30-Pro204Accession # NP_757373 |
| Source | Recombinant Monoclonal Mouse IgG1 Clone # 3316R |
| Purification | Protein A or G purified from cell culture supernatant |
| Specificity | Detects human G-CSF in direct ELISAs. |
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Human G-CSF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human G‑CSF Antibody (Catalog #MAB214R ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human G‑CSF Biotinylated Antibody (Catalog #BAF214 ) |
| ELISA Standard | Recombinant Human G-CSF Protein (Catalog #214-CS ) | |
| Neutralization | Measured by its ability to neutralize G-CSF-induced proliferation in the NFS-60 mouse myeloid cell line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.01-0.03 μg/mL in the presence of 0.125 ng/mL Recombinant Human G-CSF. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization G-CSF Antibody (3316R) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/GCSF_MAB214R_Block_Neutralize_19711.jpg) click image to view larger | Cell Proliferation Induced by G-CSF and Neutralization by Human G-CSF Antibody.Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human G-CSF Monoclonal Antibody (Catalog # MAB214R). The ND50 is typically 0.01-0.03 μg/mL. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after opening. | ||
| 6 months, -20 to -70 °C under sterile conditions after opening. | ||
| Background: G-CSF | G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. |
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| Long Name: | Granulocyte Colony Stimulating Factor | |
| Entrez Gene IDs: | 1440 (Human); 12985 (Mouse) | |
| Alternate Names: | C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin | |
追加しました。
Related Research Areas
| ALS Related Molecules |
| Cytokines and Receptors in Angiogenesis |
| Gametes and Fertilization |
| IL-6 Family |
| Macrophage Activation Markers |
| Neutralization | |
|---|---|
 | Cell Proliferation Induced by G-CSF and Neutralization by Human G-CSF Antibody. Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human G-CSF Monoclonal Antibody (Catalog # MAB214R). The ND50 is typically 0.01-0.03 μg/mL. |
追加しました。
Related Product & Information
| Long Name | Granulocyte Colony Stimulating Factor |
|---|---|
| Entrez Gene IDs | 1440 (Human); 12985 (Mouse) |
| Background | G-CSF |
| background_content | Background: G-CSF G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. |
追加しました。
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