Mouse/Rat IL-17F Quantikine ELISA Kit

R&D Systems製のMouse/Rat IL-17Fを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。

The Quantikine Mouse/Rat IL-17F Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-17F in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-17F and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-17F showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-17F.

The recovery of mouse IL-17F spiked to levels throughout the range of the assay in various matrices was evaluated.

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17F in each matrix were diluted with Calibrator Diluent and assayed.

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.