Human Serpin C1/Antithrombin-III Quantikine ELISA Kit
掲載日情報：2016/08/18 現在Webページ番号：177609
R&D Systems製のHuman Serpin C1/Antithrombin-IIIを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月02日 20時55分現在]
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Human Serpin C1/Antithrombin-III Quantikine ELISA Kit |
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[在庫・価格 ：2024年05月02日 20時55分現在]
Human Serpin C1/Antithrombin-III Quantikine ELISA Kit
文献数： 1
- 商品コード：DSPC10
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別売品コントロール：QC208，感度：0.097 ng/mL，測定範囲：0.31 - 20 ng/mL，測定試料：Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (25 uL), Human Milk (10 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Sandwich，アッセイ数：96，測定時間：3.5 hours，測定因子：Serpin C1/Antithrombin-III 別名：Antithrombin-III Genbank No： 462 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human Serpin C1 ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
| Sensitivity | 3.5 hours |
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| Assay Length | 0.097 ng/mL |
| Assay Range | 0.313 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk,) |
| Specificity | Natural and recombinant free and complexed human Serpin C1. |
| Cross-reactivity | ＜ 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested. |
| Interference | No significant interference observed with available related molecules. |
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Product Summary
The Quantikine Human Serpin C1/Antithrombin-III Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Serpin C1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human Serpin C1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin C1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin C1.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Inter-Assay Precision (Precision between assays Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.95 | 6.37 | 12.2 | 1.84 | 5.82 | 11.8 |
| Standard Deviation | 0.077 | 0.224 | 0.303 | 0.079 | 0.432 | 0.778 |
| CV% | 3.9 | 3.5 | 2.5 | 4.3 | 7.4 | 6.6 |
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Recovery
The recovery of human Serpin C1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 93 | 83-108 |
| Urine (n=4) | 99 | 92-109 |
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Lenearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin C1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Background
The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
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Additional Infomation
| Entrez Gene IDs | 462 (Human); 11905 (Mouse); |
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| Alternate Names | Antithrombin-III; ATIII; AT3antithrombin-III; ATIIIantithrombin III; MGC22579; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serine-cysteine proteinase inhibitor clade C member 1; Serpin C1; serpin peptidase inhibitor, clade C (antithrombin), member 1; |
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