Human Hepcidin Quantikine ELISA Kit

掲載日情報:2016/08/18 現在Webページ番号:177607

R&D Systems製のHuman Hepcidinを測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年04月26日 08時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Human Hepcidin Quantikine ELISA Kit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 3
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説明文
感度:3.81 pg/mL,測定範囲:15.6-1,000 pg/mL,測定試料:Serum, Plasma, Cell Culture Supernatant, Urine,種交差性:Human,検出方法:colorimetric,測定波長:450 nm,アッセイ法:Sandwich,アッセイ数:96,別売品コントロール:QC220,測定時間:4.5 hours,測定因子:Hepcidin
別名:HAMP
Genbank No: 57817
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Quantikine Human Hepcidin ELISA Kit
ELISA Kit製品に関するFAQ(R&D Systems)
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集
Quantikine ELISA Kit

[在庫・価格 :2025年04月26日 08時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Human Hepcidin Quantikine ELISA Kit

文献数: 3

説明文 感度:3.81 pg/mL,測定範囲:15.6-1,000 pg/mL,測定試料:Serum, Plasma, Cell Culture Supernatant, Urine,種交差性:Human,検出方法:colorimetric,測定波長:450 nm,アッセイ法:Sandwich,アッセイ数:96,別売品コントロール:QC220,測定時間:4.5 hours,測定因子:Hepcidin
別名:HAMP
Genbank No: 57817
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Quantikine Human Hepcidin ELISA Kit
ELISA Kit製品に関するFAQ(R&D Systems)
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集
Quantikine ELISA Kit

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Product Details

Sensitivity 4.5 hours
Assay Length 3.81 pg/mL
Assay Range 5.60 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine,)
Specificity Natural human Hepcidin.
Cross-reactivity < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

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Product Summary

The Quantikine Human Hepcidin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Hepcidin in cell culture supernates, serum, plasma, and urine. It contains synthetic Hepcidin and antibodies raised against synthetic Hepcidin. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring human Hepcidin.

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Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Inter-Assay Precision (Precision between assays Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Inter-Assay Precision (Precision between assays Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 175 350 726 164 328 647
Standard Deviation 7.51 10.8 23.5 18 26.1 39.8
CV% 4.3 3.1 3.2 11 8 6.2
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 175 350 726 164 328 647
Standard Deviation 7.51 10.8 23.5 18 26.1 39.8
CV% 4.3 3.1 3.2 11 8 6.2

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Recovery

The recovery of human Hepcidin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 111 99-118
EDTA Plasma (n=4) 103 95-115
Heparin Plasma (n=4) 104 93-117
Serum (n=4) 106 96-118

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Lenearity

To assess the linearity of the assay, samples containing high concentrations of human Hepcidin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

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Additional Infomation

Entrez Gene IDs 57817 (Human);
Alternate Names HAMP; HEPC; HFE2B; LEAP1; PLTR; hepcidin antimicrobial peptide; HEPCPutative liver tumor regressor; HFE2Bhepcidin; LEAP-1; LEAP1PLTR; Liver-expressed antimicrobial peptide 1;

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お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

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