抗Mouse Erythropoietin抗体(Anti-Mouse Erythropoietin antibody)
掲載日情報:2021/01/28 現在Webページ番号:177547
Mouse Erythropoietinに対する抗体(Anti-Mouse Erythropoietin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 :2024年05月20日 13時35分現在]
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Anti-Mouse Erythropoietin MAb (Clone 148436) |
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Anti-Mouse Erythropoietin MAb (Clone 148436) |
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Anti-Mouse Erythropoietin MAb (Clone 148436) |
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[在庫・価格 :2024年05月20日 13時35分現在]
Anti-Mouse Erythropoietin MAb (Clone 148436)
文献数: 0
- 商品コード:MAB9591-100
- メーカー:RSD
- 包装:100μg
- 価格:¥97,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
別名:EP クローン:148436 Genbank No: 2056 Protein Accession No: P07321 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
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関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse Erythropoietin MAb (Clone 148436)
文献数: 0
- 商品コード:MAB9591-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Mouse Erythropoietin サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAM9592,スタンダードとして#959-ME-010を用いる。 別名:EP クローン:148436 Genbank No: 2056 Protein Accession No: P07321 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
|
||||||
製品記事 |
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
||||||
関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse Erythropoietin MAb (Clone 148436)
文献数: 0
- 商品コード:MAB9591-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
※受注発注品。形状:溶液または凍結乾燥 別名:EP クローン:148436 Genbank No: 2056 Protein Accession No: P07321 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
|
||||||
製品記事 |
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
||||||
関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
追加しました。
Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | Chinese hamster ovary cell line CHO-derived recombinant mouse Erythropoietin Ala27-Arg192Accession # P07321 |
Source | Monoclonal Rat IgG2A Clone # 148436 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse Erythropoietin in direct ELISAs. In direct ELISAs, approximately 25-50%cross-reactivity with recombinant rat Erythropoietin, 5-15% cross-reactivitywith recombinant human Erythropoietin, and no cross-reactivity with recombinantmouse Thrombopoietin is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Mouse Erythropoietin Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse Erythropoietin Antibody (Catalog #MAB9591 ) |
ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Mouse Erythropoietin Biotinylated Antibody (Catalog #BAM9592 ) |
ELISA Standard | Recombinant Mouse Erythropoietin Protein (Catalog #959-ME ) | |
Neutralization | Measured by its ability to neutralize Erythropoietin-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 10 ng/mL Recombinant Mouse Erythropoietin. | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Neutralization |
click image to view larger | Cell Proliferation Induced by Erythropoietin and Neutralization by Mouse Erythropoietin Antibody.Recombinant Mouse Erythropoietin (Catalog # 959‑ME) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Erythropoietin Monoclonal Antibody (Catalog # MAB9591). The ND50 is typically 0.15‑0.75 µg/mL. |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: Erythropoietin | Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N‑glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of Epo is required for biological activities in vivo (4). Mature mouse Epo shares 95% amino acid sequence identity with rat Epo and 73%‑82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6‑8). Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R) (8, 9). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). Epo additionally plays a tissue‑protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13). |
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References
Koury, M. J. (2005) Exp. Hematol. 33:1263. | |
Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849. | |
Wen, D. et al. (1993) Blood 82:1507. | |
Tsuda E. et al. (1990) Eur. J. Biochem. 188:405. | |
Lacombe, C. et al. (1988) J. Clin. Invest. 81:620. | |
Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13. | |
Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184. | |
Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025. | |
Koury, M.J. and M.C. Bondurant (1990) Science 248:378. | |
Acs, G. et al. (2001) Cancer Res. 61:3561. | |
Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332. | |
Verdier, F. et al. (2000) J. Biol. Chem. 275:18375. | |
Kertesz, N. et al. (2004) Dev. Biol. 276:101. | |
Entrez Gene IDs: | 2056 (Human); 13856 (Mouse); 24335 (Rat) |
Alternate Names: | EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 |
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Related Research Areas
Cytokine and Growth Factor Receptors on VSMC | ||||||||
HIF Transcription Factors | ||||||||
Red Blood Cells (RBCs) | ||||||||
Renal Markers | ||||||||
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Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
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Excellent | IHC | Mouse | Anonymous | 04/13/2018 |
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Summary
Application | Immunohistochemistry | |||||||
Sample Tested | Serum | |||||||
Species | Mouse | |||||||
Excellent | Affinity Purification | Mouse | Anonymous | 03/15/2016 |
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Summary
Application | Affinity Purification |
Sample Tested | 3T3-L1 mouse embryonic fibroblast adipose-like cell line |
Species | Mouse |
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Related Product & Information
Entrez Gene IDs | 2056 (Human); 13856 (Mouse); 24335 (Rat) |
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Background | Erythropoietin |
background_content | Background: Erythropoietin Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N‑glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of Epo is required for biological activities in vivo (4). Mature mouse Epo shares 95% amino acid sequence identity with rat Epo and 73%‑82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6‑8). Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R) (8, 9). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). Epo additionally plays a tissue‑protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13). |
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Citations
- FGF23 expression in rodents is directly induced via erythropoietin after inhibition of hypoxia inducible factor proline hydroxylase
Authors: I Flamme, P Ellinghaus, D Urrego, T Krüger
PLoS ONE, 2017;12(10):e0186979.
Species: Rat
Sample Type: In Vivo
Application: Neut
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