抗Mouse IL-1 beta/IL-1F2抗体(Anti-Mouse IL-1 beta/IL-1F2 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-1 beta /IL-1F2Val118-Ser269Accession # P10749
Source	Monoclonal Rat IgG1 Clone # 30311
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-1 beta /IL-1F2 in ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Mouse IL-1 beta /IL-1F2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑1 beta /IL‑1F2 Antibody (Catalog #MAB401 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog #BAF401 )
ELISA Standard		Recombinant Mouse IL-1 beta/IL-1F2 Protein (Catalog #401-ML )
Neutralization	Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral­ization by Mouse IL‑1 beta /IL‑1F2 Antibody.Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL‑1 beta.

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References

Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
Gray, P.W. et al. (1986) J. Immunol. 137:3644.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name:	Interleukin 1 beta
Entrez Gene IDs:	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:	catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Related Research Areas

Show

Alpha Granule Soluble Molecules
Cancer Biomarkers
Conventional/Classical Dendritic Cells
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
IL-1 Family
Infectious Diseases
Inflammatory Mediators
Isolation, Culture, and Identification of Natural Killer Cells
LTi Cells
Macrophage Activation Markers
Multiple Sclerosis and Myelin Related Molecules
Myeloid-derived Suppressor Cells (MDSC)
NCR-ILC3
Neuroinflammation
NOD-like Receptors and the Inflammasome
Regulatory T Cells (Tregs)
T Cell Cytokine Signaling
Th17 Cells
Th9 Cells
Mucin 1 protects against severe Streptococcus pneumoniae infection Authors: P Dhar, GZ Ng, EM Dunne, P Sutton Virulence, 2017;0(0):0. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Capture
Images	Ratings	Applications	Species	Reviewed By	Date	Details
	Excellent		ELISA	Human	Anonymous	05/17/2018

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Summary

Application	ELISA
Sample Tested	293T human embryonic kidney cell line
Species	Human

Neutralization

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral­ization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).

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Related Product & Information

Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Background	IL-1 beta/IL-1F2
background_content	Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL‑1 beta.

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Citations

1. Mucin 1 protects against severe Streptococcus pneumoniae infection
   Authors: P Dhar, GZ Ng, EM Dunne, P Sutton
   Virulence, 2017;0(0):0.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


2. Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan
   Authors: SY Nam, KY Kim, MH Kim, JB Jang, SY Rah, HJ Chae, JM Lee, HM Kim, HJ Jeong
   Pharm Biol, 2017;55(1):1856-1862.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


3. The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
   Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
   J. Leukoc. Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


4. Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice
   Authors: Y Oh, YS Kwon, BD Jung
   Int J Med Sci, 2017;14(8):729-734.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


5. Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis
   Authors: L Zhang, Z Tian, W Li, X Wang, Z Man, S Sun
   Biosci. Rep., 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


6. Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation
   Authors: Yoonsook Kim
   Pharmacogn Mag, 2016;12(48):295-301.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


7. Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice
   Authors: C Dempsey
   Brain Behav. Immun, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


8. Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
   Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
   J Immunol, 2015;194(1):334-48.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Development


9. AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
   Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
   Proc Natl Acad Sci U S A, 2015;112(22):E2891-9.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


10. Multiple CD11c+ cells collaboratively express IL-1beta to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth.
    Authors: Benahmed F, Chyou S, Dasoveanu D, Chen J, Kumar V, Iwakura Y, Lu T
    J Immunol, 2014;192(9):4153-63.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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