抗Human IFN-gamma抗体(Anti-Human IFN-gamma antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IFN-gamma Gln24-Gln166Accession # AAP20098.1
Source	Monoclonal Mouse IgG2A Clone # 25718
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IFN-gamma.

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Applications and Data

	Recommended Concentration	Sample
Immunocytochemistry	8-25 µg/mL	See below
Neutralization	Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 1 ng/mL Recombinant Human IFN‑ gamma.

Neutralization
	IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285). The ND50 is typically 0.02-0.06 µg/mL.
Immunocytochemistry
	IFN‑ gamma in Human PBMCs. IFN‑ gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Background	IFN-gamma
background_content	Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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Citations

1. DDIT4 and Associated lncDDIT4 Modulate Th17 Differentiation through the DDIT4/TSC/mTOR Pathway
   Authors: F Zhang, G Liu, D Li, C Wei, J Hao
   J. Immunol., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


2. PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2
   Authors: Y Liu, L Qu, Y Liu, B Roizman, GG Zhou
   Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Bilirubin suppresses Th17 immunity in colitis by upregulating CD39
   Authors: MS Longhi, M Vuerich, A Kalbasi, JE Kenison, A Yeste, E Csizmadia, B Vaughn, L Feldbrugge, S Mitsuhashi, B Wegiel, L Otterbein, A Moss, FJ Quintana, SC Robson
   JCI Insight, 2017;2(9):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Production of Proinflammatory Cytokines by Monocytes in Liver-Transplanted Recipients with De Novo Autoimmune Hepatitis Is Enhanced and Induces TH1-like Regulatory T Cells
   Authors: AS Arterbery, A Osafo-Addo, Y Avitzur, M Ciarleglio, Y Deng, SJ Lobritto, M Martinez, DA Hafler, M Kleinewiet, UD Ekong
   J. Immunol., 2016;196(10):4040-51.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Innate Invariant NKT Cell Recognition of HIV-1-Infected Dendritic Cells Is an Early Detection Mechanism Targeted by Viral Immune Evasion
   J Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC - Fresh


6. Modulation of Stat-1 in Human Macrophages Infected with Different Species of Intracellular Pathogenic Bacteria
   J Immunol Res, 2016;2016(0):5086928.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
   Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil
   Nat Immunol, 2015;16(3):318-25.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
   Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
   PLoS ONE, 2014;9(5):e96695.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation.
   Authors: Chang HC, Sehra S, Goswami R, Yao W, Yu Q, Stritesky GL, Jabeen R, McKinley C, Ahyi AN, Han L, Nguyen ET, Robertson MJ, Perumal NB, Tepper RS, Nutt SL, Kaplan MH
   Nat. Immunol., 2010;11(6):527-34.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Impaired development of human Th1 cells in patients with deficient expression of STAT4.
    Authors: Chang HC, Han L, Goswami R, Nguyen ET, Pelloso D, Robertson MJ, Kaplan MH
    Blood, 2009;113(23):5887-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Other: Cell differentiation

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