抗Human IL-4 R alpha抗体(Anti-Human IL-4 R alpha antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 RGly24-His232Accession # P24394
Source	Monoclonal Mouse IgG2A Clone # 25463
Purification	Protein A or G purified from ascites
Specificity	Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑4 R alpha (Catalog # 230-4R) under non-reducing conditions only
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 3-6 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.

Flow Cytometry
	Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunohistochemistry
	IL‑4 R alpha in Human Lymph Node. IL‑4 R alpha was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha  Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230). The ND50 is typically 3-6 ng/mL.

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Related Product & Information

Long Name	Interleukin 4 Receptor alpha
Entrez Gene IDs	3566 (Human); 16190 (Mouse)
Background	IL-4 R alpha
background_content	Background: IL-4 R alpha Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.

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Citations

1. Allergen-specific IgG antibodies signaling via Fc?RIIb promote food tolerance
   Authors: OT Burton, JM Tamayo, AJ Stranks, KJ Koleoglou, HC Oettgen
   J. Allergy Clin. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


2. IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
   Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
   Cancer Res., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
   Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
   J Immunol, 2014;192(1):523-32.
   Species: Human
   Sample Type: Whole Cells
   Application: IF


4. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
   Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
   PLoS ONE, 2014;9(5):e96925.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Absence of a role for interleukin-13 in inflammatory bowel disease.
   Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
   Eur J Immunol, 2014;44(2):370-85.
   


6. IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
   Authors: Reece P, Gauvreau G, Sehmi R, Denburg J
   PLoS ONE, 2014;9(6):e100734.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Platelet ITAM signaling is critical for vascular integrity in inflammation.
   Authors: Boulaftali, Yacine, Hess, Paul R, Getz, Todd M, Cholka, Agnieszk, Stolla, Moritz, Mackman, Nigel, Owens, A Philli, Ware, Jerry, Kahn, Mark L, Bergmeier, Wolfgang
   J Clin Invest, 2013;123(2):908-16.
   Species: Human
   Sample Type: Whole Cells
   Application: Binding Assay


8. Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide.
   Authors: Sarangthem, Vijaya, Cho, Eun A, Bae, Sang Mun, Singh, Thoudam, Kim, Sun-Ji, Kim, Soyoun, Jeon, Won Bae, Lee, Byung-He, Park, Rang-Woo
   PLoS ONE, 2013;8(12):e81891.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


9. Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting.
   Authors: Amano Y, Yamashita Y, Kojima K, Yoshino K, Tanaka N, Sugamura K, Takeshita T
   J. Biol. Chem., 2011;286(17):15458-72.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4.
    Authors: Thors L, Bergh A, Persson E, Hammarsten P, Stattin P, Egevad L, Granfors T, Fowler CJ
    PLoS ONE, 2010;5(8):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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