抗Human IL-13抗体(Anti-Human IL-13 antibody)
掲載日情報：2021/01/28 現在Webページ番号：177223
Human IL-13に対する抗体(Anti-Human IL-13 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年05月10日 00時01分現在]
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Anti-Human IL-13 MAb (Clone 32116) |
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Anti-Human IL-13 MAb (Clone 32116) |
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Anti-Human IL-13 MAb (Clone 32116) |
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[在庫・価格 ：2025年05月10日 00時01分現在]
Anti-Human IL-13 MAb (Clone 32116)
文献数： 1
- 商品コード：MAB213-100
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：ALRHMGC116789 クローン：32116 Genbank No： 3596 Protein Accession No： P35225 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human IL-13 MAb (Clone 32116)
文献数： 1
- 商品コード：MAB213-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human IL-13 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF213，スタンダードとして#213-ILB-005を用いる。 別名：ALRHMGC116789 クローン：32116 Genbank No： 3596 Protein Accession No： P35225 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human IL-13 MAb (Clone 32116)
文献数： 1
- 商品コード：MAB213-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：ALRHMGC116789 クローン：32116 Genbank No： 3596 Protein Accession No： P35225 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human IL-13Gly35-Asn146Accession # P35225 |
| Source | Monoclonal Mouse IgG1 Clone # 32116 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human IL-13 in direct ELISAs. |
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Applications and Data
| Recommended Concentration | Sample | |
| Flow Cytometry | 2.5 µg/106 cells | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Human IL-13 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human IL‑13 Antibody (Catalog #MAB213 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human IL‑13 Biotinylated Antibody (Catalog #BAF213 ) |
| ELISA Standard | Recombinant Human IL-13 Protein (Catalog #213-ILB ) | |
| Neutralization | Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.15-0.75 ug/mL in the presence of 10 ng/mL Recombinant Human IL‑13. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Flow Cytometry |
 click image to view larger | Detection of IL‑13 in Human PBMCs by Flow Cytometry.Human peripheral blood mononuclear cells (PBMCs) treated with 5 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL‑13 Monoclonal Antibody (Catalog # MAB213) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). |
| Neutralization |
 click image to view larger | Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 Antibody.Recombinant Human IL‑13 (Catalog # 213-ILB) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑13 Monoclonal Antibody (Catalog # MAB213). The ND50 is typically 0.15‑0.75 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-13 | IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17). |
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References
| Wills-Karp, M. (2004) Immunol. Rev. 202:175. | |
| Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265. | |
| McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735. | |
| Moy, F.J. et al. (2001) J. Mol. Biol. 310:219. | |
| Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231. | |
| Ruetten, H. and C. Thiemermann (1997) Shock 8:409. | |
| Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529. | |
| Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425. | |
| Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073. | |
| Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338. | |
| Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687. | |
| Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387. | |
| Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464. | |
| Tabata, Y. et al. (2007) J. Immunol. 177:7905. | |
| Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858. | |
| Joshi, B.H. et al. (2006) Vitam. Horm. 74:479. | |
| Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91. | |
| Long Name: | Interleukin 13 |
| Entrez Gene IDs: | 3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate) |
| Alternate Names: | ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600 |
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Related Research Areas
| Cytokines and Receptors in Allergy and Asthma | ||||||||
| Cytokines and Receptors in Angiogenesis | ||||||||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||||||||
| gamma delta T Cells | ||||||||
| Group 2 ILCs | ||||||||
| Myeloid-derived Suppressor Cells (MDSC) | ||||||||
| Natural Killer T (NKT) Cells | ||||||||
| T Cell Cytokine Signaling | ||||||||
| Th2 Cells | ||||||||
| Th22 Cells | ||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
|---|---|---|---|---|---|---|---|---|
| Excellent | ELISA | Human | Anonymous | 01/18/2018 | ||||
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Summary
| Application | ELISA | |||||||
| Sample Tested | EDTA Plasma | |||||||
| Species | Human | |||||||
| Excellent | ICC/IF | Hamster | Anonymous | 12/13/2017 |
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Summary
| Application | Immunocytochemistry/Immunofluorescence |
| Sample Tested | CHO Chinese hamster ovary cell line |
| Species | Hamster |
| Flow Cytometry | |
|---|---|
 | Detection of IL‑13 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 5 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL‑13 Monoclonal Antibody (Catalog # MAB213) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). |
| Neutralization | |
 | Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 Antibody. Recombinant Human IL‑13 (Catalog # 213-ILB) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑13 Monoclonal Antibody (Catalog # MAB213). The ND50 is typically 0.15‑0.75 µg/mL. |
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Related Product & Information
| Long Name | Interleukin 13 |
|---|---|
| Entrez Gene IDs | 3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate) |
| Background | IL-13 |
| background_content | Background: IL-13 IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17). |
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Citations
- Cytokine milieu of atopic dermatitis skin subverts the innate immune response to vaccinia virus.
Authors: Howell MD, Gallo RL, Boguniewicz M, Jones JF, Wong C, Streib JE, Leung DY
Immunity, 2006;24(3):341-8.
Species: Human
Sample Type: Whole Cells
Application: Neut - Th2 cytokine response in Major Depressive Disorder patients before treatment.
Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
J. Neuroimmunol., 2006;172(1):156-65.
Species: Human
Sample Type: Serum
Application: ELISA Development
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