抗Human B7-1/CD80抗体(Anti-Human B7-1/CD80 antibody)
掲載日情報：2021/01/28 現在Webページ番号：177166
Human B7-1/CD80に対する抗体(Anti-Human B7-1/CD80 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human B7-1/CD80 MAb (Clone 37711) |
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Anti-Human B7-1/CD80 MAb (Clone 37711) |
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Anti-Human B7-1/CD80 MAb (Clone 37711) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human B7-1/CD80 MAb (Clone 37711)
文献数： 1
- 商品コード：MAB140-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human B7-1/CD80 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAM1402，スタンダードとして#140-B1-100を用いる。 別名：Activation B7-1 antigen クローン：37711 Genbank No： 941 Protein Accession No： P33681 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IHC,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human B7-1/CD80 MAb (Clone 37711)
文献数： 1
- 商品コード：MAB140-100
- メーカー：RSD
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：Activation B7-1 antigen クローン：37711 Genbank No： 941 Protein Accession No： P33681 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IHC,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human B7-1/CD80 MAb (Clone 37711)
文献数： 1
- 商品コード：MAB140-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：Activation B7-1 antigen クローン：37711 Genbank No： 941 Protein Accession No： P33681 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IHC,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80Val35-Asn242 (predicted)Accession # P33681 |
| Source | Monoclonal Mouse IgG1 Clone # 37711 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Flow Cytometry | 2.5 µg/106 cells | See below |
| Immunohistochemistry | 8-25 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Human B7-1/CD80 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human B7‑1/CD80 Antibody (Catalog #MAB140 ) |
| ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Human B7‑1/CD80 Biotinylated Antibody (Catalog #BAM1402 ) |
| ELISA Standard | Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF (Catalog #140-B1 ) | |
| Neutralization | Measured by its ability to neutralize B7‑1/CD80 and PHA-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera and 10 µg/mL PHA. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Flow Cytometry |
![Flow Cytometry B7-1/CD80 Antibody (37711) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/B71_MAB140_Flow_Cytometry_16025.jpg) click image to view larger | Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry.Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). |
| Neutralization |
![Neutralization B7-1/CD80 Antibody (37711) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/B7-1_MAB140_Block_Neutralize_9024.jpg) click image to view larger | IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Human B7‑1/CD80 Antibody. Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Human B7‑1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND50 is typically 0.2-1 µg/mL. |
| Immunohistochemistry |
![Immunohistochemistry B7-1/CD80 Antibody (37711) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/B71_MAB140_Immunohistochemistry_17363.jpg) click image to view larger | B7‑1/CD80 in Human Tonsil. B7‑1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: B7-1/CD80 | B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through Interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequenceidentity. Human and mouse B7-1 share 44% aa sequenceidentity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites. |
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References
| Azuma, M. et al. (1993) Nature 366:76. | |
| Freeman, G.J. et al. (1993) Science 262:909. | |
| Freeman, G. et al. (1991) J. Exp. Med. 174:625. | |
| Selvakumar, A. et al. (1993) Immunogenetics 38:292. | |
| Chen, C. et al. (1994) J. Immunol. 152:4929. | |
| Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185. | |
| Entrez Gene IDs: | 941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey) |
| Alternate Names: | Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80 |
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Related Research Areas
| B Cell Activation | ||
| B Cell Markers | ||
| Common Macrophage Markers | ||
| Co-stimulatory and Co-inhibitory Molecules | ||
| Co-stimulatory Molecules Associated with Tfh Cells | ||
| Dendritic Cell Co-stimulatory Molecules | ||
| Dendritic Cell Development | ||
| Macrophage Activation Markers | ||
| Mouse Tissue-Resident Macrophage Markers | ||
| Multiple Myeloma | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Regulatory T Cells (Tregs) | ||
| T Cell Antigen Recognition | ||
| ||
| Flow Cytometry | |
|---|---|
 | Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). |
| Neutralization | |
 | IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Human B7‑1/CD80 Antibody. Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Human B7‑1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND50 is typically 0.2-1 µg/mL. |
| Immunohistochemistry | |
 | B7‑1/CD80 in Human Tonsil. B7‑1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Related Product & Information
| Entrez Gene IDs | 941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey) |
|---|---|
| Background | B7-1/CD80 |
| background_content | Background: B7-1/CD80 B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through Interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequenceidentity. Human and mouse B7-1 share 44% aa sequenceidentity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- Tubular B7-1 expression parallels proteinuria levels, but not clinical outcomes in adult minimal change disease patients
Authors: SW Lee, SH Baek, JH Paik, S Kim, KY Na, DW Chae, HJ Chin
Sci Rep, 2017;7(0):41859.
Species: Human
Sample Type: Whole Tissue
Application: IHC - paraffin - Autoantibodies against CD80 in patients with COPD
Authors: Xiongbiao Wang
Clin Transl Immunology, 2016;5(10):e103.
Species: Human
Sample Type: Serum
Application: WB - Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
J Immunol Res, 2014;2014(0):593562.
Species: Human
Sample Type: Whole Cells
Application: Functional Assay - B7-CD28 costimulatory signals control the survival and proliferation of murine and human gammadelta T cells via IL-2 production.
J. Immunol., 2012;189(3):1202-8.
Species: Human
Sample Type: Whole Cells
Application: Neut - ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Blood, 2012;119(26):6268-77.
Species: Human
Sample Type: Whole Cells
Application: Neut - A synergistic antitumor effect of interleukin-2 addition with CD80 immunogene therapy for peritoneal metastasis of gastric carcinoma.
Authors: Kosaka K, Yashiro M, Sakate Y, Hirakawa K
Dig. Dis. Sci., 2007;52(8):1946-53.
Species: Human
Sample Type: Whole Cells
Application: Neut
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