抗Human CD14PerCP抗体(Anti-Human CD14PerCP antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	PerCP (Peridinin-chlorophyll Protein Complex)
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human CD14Thr20-Cys352Accession # P08571
Source	Monoclonal Mouse IgG1 Clone # 134620
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CD14 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of CD14 in Human PBMCs byFlow Cytometry. Human peripheral blood mononuclearcells (PBMCs) were stained with Mouse Anti-Human CD14 PerCP‑conjugatedMonoclonal Antibody (Catalog # FAB3832C). View our protocol for StainingMembrane-associated Proteins.

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Related Product & Information

Background

CD14

background_content

Background: CD14 CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

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Citations

1. Human Monocyte Subsets Are Transcriptionally and Functionally Altered in Aging in Response to Pattern Recognition Receptor Agonists
   Authors: TU Metcalf, PA Wilkinson, MJ Cameron, K Ghneim, C Chiang, AM Wertheimer, JB Hiscott, J Nikolich-Z, EK Haddad
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Global analyses revealed age-related alterations in innate immune responses after stimulation of pathogen recognition receptors.
   Authors: Metcalf T, Cubas R, Ghneim K, Cartwright M, Grevenynghe J, Richner J, Olagnier D, Wilkinson P, Cameron M, Park B, Hiscott J, Diamond M, Wertheimer A, Nikolich-Zugich J, Haddad E
   Aging Cell, 2015;14(3):421-32.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Immune activation alters cellular and humoral responses to yellow fever 17D vaccine.
   Authors: Muyanja E, Ssemaganda A, Ngauv P, Cubas R, Perrin H, Srinivasan D, Canderan G, Lawson B, Kopycinski J, Graham A, Rowe D, Smith M, Isern S, Michael S, Silvestri G, Vanderford T, Castro E, Pantaleo G, Singer J, Gillmour J, Kiwanuka N, Nanvubya A, Schmidt C, Birungi J, Cox J, Haddad E, Kaleebu P, Fast P, Sekaly R, Trautmann L, Gaucher D
   J Clin Invest, 2014;124(7):3147-58.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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