抗CtIP [19E8]抗体 | Anti-CtIP [19E8] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

CtIP antibody [19E8] detects CtIP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CtIP protein stained by CtIP antibody [19E8] (GTX70264) diluted at 1:100.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	19E8
Isotype	IgG1
Application	WB, ICC/IF, IP, ChIP assay, PLA
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:1000
IP	Assay dependent
ChIP assay	Assay dependent
PLA	Assay dependent
Note : WB 3% Milk/TBST overnight

Not tested in other applications.

Calculated MW	125 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.24 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human CtIP
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Rb Binding Protein 8, Endonuclease , Com1 , Ctip , Jwds , Rim , Sae2 , Sckl2 , Rbbp8
Background	CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disruppted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation.
Database	・ Gene ID: 5932 ・ UniProt: Q99708

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Ahrabi S et al. Nucleic Acids Res 2016; (Epub) A role for human homologous recombination factors in suppressing microhomology-mediated end joining.	Application : WB Reactivity : Human
Baude A et al. Nucleic Acids Res 2015; (Epub) Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination.	Application : PLA Reactivity : Human
Liao CC et al. J Biol Chem 2010; 285 (43) : 33134-43 RB·E2F1 complex mediates DNA damage responses through transcriptional regulation of ZBRK1.	Application : WB Reactivity : Human
Gu B et al. Cell Cycle 2009; 8 (9) : 1409-20 Expression of PCNA-binding domain of CtIP, a motif required for CtIP localization at DNA replication foci, causes DNA damage and activation of DNA damage checkpoint.	Application : ICC/IF Reactivity : Human
Wu M et al. Mol Cancer Res 2007; 5 (12) : 1285-95 ctip silencing as a novel mechanism of * resistance in breast cancer.	Application : WB Reactivity : Human

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