抗Carbonic Anhydrase IX [GT12]抗体 | Anti-Carbonic Anhydrase IX [GT12] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCE

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
	Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
	Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200μM CoCl2 (pink) for 48 hr.
	Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
	Carbonic Anhydrase IX antibody [GT12] detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human cervical carcinoma.Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
	The IHC-P analysis of Carbonic Anhydrase IX antibody [GT12] was published by Huang WJ and colleagues in the journal PLoS One in 2015.PMID: 25738958
	Sample (30 μg HeLa whole cell lysate) A: 24 hr Untreated B: 24 hr treatment with 100μM CoCl2 C: 24 hr treatment with 200μM CoCl2 D: 48 hr Untreated E: 48 hr treatment with 100μM CoCl2 F: 48 hr treatment with 200μM CoCl2 Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200μM CoCl2 for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	GT12
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
Reactivity	Human

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APPLICATION

Application Note

For ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:100-1:1000. For IHC-P: Use at a dilution of 1:100-1:1000. For FACS: Use at a dilution of 1:100-1:1000 for 1x10^6 cells. For IP: Use at a concentration of 2-10μg/mg lysate. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW	50 kDa. ( Note )
Specificity/Sensitivity	Clone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Carbonic Anhydrase 9 , Caix , Mn , Ca9
Background	Carbonic anhydrase IX (CA IX) is a member of the CA family of zinc-binding enzymes that catalyze a reversible conversion between carbon dioxide and carbonic acid, in a reaction that involves facilitated hydration of CO2 to H2CO3 followed by the spontaneous dissociation of H2CO3 into bicarbonate and proton. The CA IX molecule consists of a large extracellular domain (ECD), single-pass transmembrane region (TM) and a short intracytoplasmic (IC) tail. The ECD contains an N-terminally located PG-like region (which is absent from the other carbonic anhydrase isoforms) and a centrally located, well conserved catalytic domain (CA). CA IX is a cell surface protein that is present in human tumors, but not in the corresponding normal tissues. Moreover, expression of CA IX correlates with poor prognosis in many tumor types. CA IX plays a role in two phenomena involved in development of tumor phenotype - control of cell adhesion and pH regulation. Tight association of CA IX with tumors is to a major part related to tumor hypoxia.
Database	・ Gene ID: 768 ・ UniProt: Q16790

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REFERENCE

Application Reference	Application/Reactivity
Davis RM et al. ACS Nano 2018; 12 (10) : 9669-9679 Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype.
Thompson JM et al. Mol Cancer Ther 2018; (Epub) Targeting the mevalonate pathway suppresses VHL-deficient CC-RCC through a HIF-dependent mechanism.	Application : WB Reactivity : Human
Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1) : 70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.	Application : WB Reactivity : Human
Ramchandani D et al. Lab Invest 2016; 96 (12) : 1234-1245 Activation of carbonic anhydrase IX by alternatively spliced tissue factor under late-stage tumor conditions.	Application : WB Reactivity : Human
Abd-Aziz N et al. Oncol Lett 2015; 10 (4) : 2192-2196 * attenuates hif-1- but not hif-2-mediated transcriptional activation.	Application : WB Reactivity : Human

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