抗TET1 [GT1462]抗体 | Anti-TET1 [GT1462] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	TET1 antibody [GT1462] detects TET1 protein by western blot analysis.A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells6% SDS-PAGETET1 antibody [GT1462] (GTX627420) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 antibody [GT1462] (GTX627420) dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
	TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GTX627420) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
	NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GTX627420) diluted at 1:500.
	Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 μg of TET1 antibody [GT1462] (GTX627420).Western blot analysis was performed using TET1 antibody [GT1462] (GTX627420) diluted at 1:500.EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
	TET1 antibody detects TET1 protein by Western blot analysis.A. 30 μg 293T whole cell lysate/extractB. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells5 % SDS-PAGETET1 antibody (GTX627420) dilution: 1:500

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1462
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW	235 kDa. ( Note )
Positive Control	293T , DDDDK-human TET1-transfected 293T , NT2D1 , NT2D1 nuclear extract

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.
Purification	Affinity purified by Protein G.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1
Cellular Localization	Nucleus
Background	Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.
Database	・ Gene ID: 80312 TET1 ・ UniProt: Q8NFU7 TET1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Chen LY et al. J Pathol 2019; (Epub) TET1 reprograms the epithelial ovarian cancer epigenome and reveals casein kinase 2α as a therapeutic target.
Huang Y et al. Int J Mol Sci 2019; 20 (8) TET1 Knockdown Inhibits Porphyromonas gingivalis LPS/IFN-γ-Induced M1 Macrophage Polarization through the NF-κB Pathway in THP-1 Cells.	Application : WB Reactivity : Human
Su PH et al. Cancer Lett 2019; 450 : 53-62 TET1 promotes 5hmC-dependent stemness, and inhibits a 5hmC-independent epithelial-mesenchymal transition, in cervical precancerous lesions.	Application : WB, IP, ChIP assay Reactivity : Human
Li M et al. Oncogene 2019; (Epub) Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2.	Application : WB Reactivity : Human
Chatterjee B et al. Biochim Biophys Acta Gene Regul Mech 2018; 1861 (11) : 1048-1061 DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.	Application : WB Reactivity : Mouse

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