抗Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) - ChIP grade抗体 | Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) - ChIP grade antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	ChIP was performed with HeLa nuclear extract and either 1 μl of H3K27me3S28p antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to RPL30 promoter or MyoD.
	ELISA was performed using a serial dilution of Histone H3K27me3S28ph (tri-Methyl Lys27, phospho Ser28) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,300.
	Dot blot analysis of peptides containing modified histone H3 and H4 and unmodified sequences of histone H3 using H3K27me3S28ph antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptides were spotted on a membrane for analysis.
	HeLa cells were treated with colcemid to block the cell cycle in metaphase and were fixed with formaldehyde. Chromatin from 10,000 cells was sheared and used for immunoprecipitation (IP). IP was performed with 5 μl of H3K27me3S28ph antibody. The immunoprecipitated proteins were analysed by WB with the antibody diluted 1:500 in TBS-Tween containing 5% skimmed milk. Lane 1 shows the result of the IP; a negative IP control (no antibody added) and a positive control (sheared chromatin from 10,000 cells) are shown in lane 2 and 3, respectively.
	WB analysis of histone extracts (15 μg) from HeLa cells using H3K27me3S28ph antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk. Lane 2 shows the result of the WB analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature.
	ICC/IF analysis of HeLa asynchronous cells using H3K27me3S28ph antibody and DAPI. Cells were fixed with formaldehyde, permeabilized with sodium citrate and Triton X100 and blocked with PBS containing 2.5% BSA. Cells were immunofluorescently labelled with the H3K27me3S28ph antibody diluted 1:200 (green). The nuclei were stained with DAPI (blue).

目次に戻る

製品情報

Host	Rabbit
Clonality	Polyclonal
Application	WB, ICC/IF, IP, Dot, ELISA, ChIP assay
Reactivity	Human

目次に戻る

APPLICATION

Application Note

ChIP (1 μl/ChIP), ELISA (1:100 – 1:500), Dot blotting (1:20,000), Western blotting (1:250 - 1:500), Immunofluorescence (1:200), Immunoprecipitation (5 μl/IP)

目次に戻る

PROPERTIES

Form	Liquid
Buffer	Whole antiserum from rabbit containing 0.05% azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Purification	Whole antiserum from rabbit containing 0.05% azide.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms

tri Methyl Histone H3 K27 Antibody, Histone H3 phospho S28, tri-Methyl K27 Antibody, phospho S28, tri-Methyl K27 Histone H3 Antibody, histone cluster 1, H3a Antibody, H3/A Antibody, HIST1H3A Antibody, phospho Histone H3 (pS28) Antibody, triMethyl Lys27 Histone H3 Antibody, Histone H3 tri Methyl Antibody, Histone H3 (pS28) Antibody, Histone H3 triMethyl Antibody, phospho Histone H3 (pSer28) Antibody, triMethyl K27 Histone H3 Antibody, phospho Histone H3 (S28) Antibody, H3FA Antibody, triMethyl Histone H3 Antibody, Histone H3 (pSer28) Antibody, tri Methyl Histone H3 Lys27 Antibody, tri Methyl Lys27 Histone H3 Antibody, Methyl Histone H3 Antibody, Histone H3 (phospho Ser28) Antibody, tri Methyl K27 Histone H3 Antibody, Histone H3 phospho Antibody, Histone H3 Methyl Antibody, phospho Ser28, tri-Methyl Lys27 Histone H3 Antibody, Histone H3 phospho Ser28, tri-Methyl Lys27 Antibody, tri-Methyl Histone H3 Antibody, Histone H3 (phospho S28) Antibody, tri Methyl Histone H3 Antibody, Histone H3 tri-Methyl Antibody, phospho Histone H3 Antibody

目次に戻る