抗HIF2 alpha [ep190b]抗体 | Anti-HIF2 alpha [ep190b] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCE

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	FACS analysis of HepG2 cells using GTX30123 HIF2 alpha antibody [ep190b]. M1 is defined by unstained cells. Purple : primary antibody Dilution : 1:400
	WB analysis of multiple samples using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1 and 5: HepG2 without Cobalt (II) Chloride Lane 2 and 6: HepG2 with Cobalt (II) Chloride Lane 3 and 7: HepG2 normoxic Lane 4 and 8 : HepG2 hypoxic Dilution : 1-2 μg/ml
	WB analysis of HeLa cell lysate using GTX30123 HIF2 alpha antibody [ep190b].
	IHC-P analysis of human heart tissue using GTX30123 HIF2 alpha antibody [ep190b].
	WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	ep190b
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IHC
Reactivity	Human, Mouse, Rat, Hamster

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1 - 2 μg/ml
ICC/IF	Assay dependent
IHC-P	1:150 - 1:300
IHC-Fr	Assay dependent
FACS	1:400
IP	Assay dependent
ELISA	1:100 - 1:2000
ChIP assay	Assay dependent
Gel supershift assays	Assay dependent
IHC	1:150 - 1:300

Not tested in other applications.

Calculated MW	96 kDa. ( Note )
Specificity/Sensitivity	This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.

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PROPERTIES

Form	Liquid
Buffer	PBS, 1% BSA, 0.05% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73
Cellular Localization	Nucleus,Nucleus speckle
Background	This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]
Database	・ Gene ID: 2034 EPAS1 ・ UniProt: Q99814 EPAS1

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REFERENCE

Application Reference	Application/Reactivity
Nagalakshmi VK et al. Clin Sci (Lond) 2018; 132 (23) : 2519-2545 Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction.	Application : IHC-Fr Reactivity : Mouse
Zera K et al. PLoS One 2017; 12 (10) : e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.	Application : WB Reactivity : Mouse
Chen S et al. Angiogenesis 2010; 13 (1) : 59-69 VHL and PTEN loss coordinate to promote mouse liver vascular lesions.	Application : IHC-P Reactivity : Mouse
Wright TM et al. J Biol Chem 2010; 285 (17) : 12916-24 Identification of Ror2 as a hypoxia-inducible factor target in von Hippel-Lindau-associated renal cell carcinoma.	Application : WB
Myers K et al. PLoS Genet 2009; 5 (1) : e1000324 ATR and Chk1 suppress a caspase-3-dependent apoptotic response following DNA replication stress.	Application : WB Reactivity : Mouse

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