抗HIF2 alpha抗体 | Anti-HIF2 alpha antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCE

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.Blue: Hoechst 33342 staining.
	HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.Blue: Hoechst 33342 staining.
	Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis on normoxic and hypoxic nuclear rat cell lysates.
	Immunocytochemistry/Immunofluorescence: HIF-2 alpha Antibody (GTX30114) - Detection of HIF-2 Alpha (Green) in RCC4 cells using GTX30114. Nuclei (Blue) are counterstained with Hoechst 33258.
	Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using GTX30114
	Immunohistochemistry: HIF-2 alpha Antibody (GTX30114) - Hif-2 alpha immunoreactivity in human cardiac myocytes
	GTX30114 utilized in western blot with PC12 nuclear extracts, 1:1,000, 5 second exposure.
	HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 0.01 O2 treatment for 24h) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Non-transfected (-) and HIF2A-transfected (+, including Myc-DDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat, Monkey, Fish

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APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:100-1:3000. Recognizes a band at 118kDa.
For IHC: Use at a dilution of 1:100.
For ICC/IF: Use at a dilution of 1:100-1:1000.
Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW	97 kDa. ( Note )
Positive Control	Hypoxic lysates , HepG2 (1% O2 treatment for 24hr and using Urea buffer) , Myc-DDK human HIF2A-transfected 293T
Specificity/Sensitivity	Specific for HIF-2 alpha/EPAS. Does not cross react with HIF-1 alpha.

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PROPERTIES

Form	Liquid
Buffer	1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	A peptide derived from the mouse/human HIF-2 alpha.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	endothelial PAS domain protein 1 , HIF-2alpha , HIF2A , HLF , HRF , MOP2 , bHLHe73
Background	Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. (1)
Database	・ Gene ID: 13819 Epas1 ・ UniProt: P97481 Epas1

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REFERENCE

Application Reference	Application/Reactivity
Bobek J et al. Histochem Cell Biol 2019; Tuftelin and HIFs expression in osteogenesis.	Application : ICC/IF, IHC-P Reactivity : Mouse
Tatli Dogan H et al. Diagn Pathol 2018; 13 (1) : 60 Prognostic significance of the programmed death ligand 1 expression in clear cell renal cell carcinoma and correlation with the tumor microenvironment and hypoxia-inducible factor expression.	Application : IHC-P Reactivity : Human
Huang Y et al. Pulm Circ 2018; : 2.05E+15 EXPRESS: Hypoxia inducible factor 2α (HIF2α/EPAS1) is associated with development of pulmonary hypertension in severe congenital diaphragmatic hernia patients.	Application : IHC-P Reactivity : Human
Bharti SK et al. Oncotarget 2018; 9 (20) : 15326-15339 Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.	Application : WB Reactivity : Human
Bin? L et al. Oncotarget 2017; 8 (48) : 83684-83697 Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.	Application : WB Reactivity : Mouse

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