抗GFP (FITC)抗体 | Anti-GFP (FITC) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

Immunofluorescence Microscopy of GFP-GOAT-Antibody. Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP (GTX26662) was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody (GTX26662). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.

目次に戻る

製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	>1:10000
ICC/IF	1:500 - 1:2500
IHC-Fr	Assay dependent
IHC-Wm	Assay dependent
ELISA	>1:20000
IHC	Assay dependent

Not tested in other applications.

目次に戻る

PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide and 10 mg/mL Bovine Serum Albumin (BSA)
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen	The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Purification	Purified by antigen-affinity chromatography. From polyclonal serum
Conjugation	Fluorescein isothiocyanate (FITC) Absorption : 495 nm ; Emission : 528 nm. Ratio : 3.5 molecules FITC per Goat IgG molecule.
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

目次に戻る

REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Lale Evsen et al. bioRxiv 2019; (Epub) Let-7 miRNAs control auditory sensory progenitor behavior in the vertebrate inner ear.	Application : IHC-Fr
Clayton ZE et al. Biosci Rep 2018; (Epub) Induced pluripotent stem cell-derived endothelial cells promote angiogenesis and accelerate wound closure in a murine excisional wound healing model.	Application : IHC
Chan AH et al. PLoS One 2017; 12 (3) : e0174773 Evaluation of synthetic vascular grafts in a mouse carotid grafting model.
Woichansky I et al. Nat Commun 2016; 7 : 10834 Three mechanisms control E-cadherin localization to the zonula adherens.	Application : IHC
Raft S et al. Dev Biol 2014; 390 (1) : 51-67 Ephrin-B2 governs morphogenesis of endolymphatic sac and duct epithelia in the mouse inner ear.

目次に戻る