抗Rb2 / p130抗体 | Anti-Rb2 / p130 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Western Blot of Rabbit Anti-Rb2 p130 Antibody. Lane 1: HEK 293 pcDNA3. Lane 2: HEK 293 pcDNA3-Rb2wt. Lane 3: HEK 293 pcDNA3-Rb2-PM19. Load: 30 μg per lane. Primary antibody: Anti-Rb2 antibody at 1:250 for overnight at 4ºC. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 130 kDa for p130/Rb2.

Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.  The staining shows the location of pRb2/p130 in developing mouse tissue.  Other detection systems should yield similar results. Sections were cut at 5-7 μm, mounted on glass and dried overnight at 37ºC.  All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS).  PBS was used for all subsequent washes and for antiserum dilution.  Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum.  Slides were incubated at 20º C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min.  Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20º C.  Diaminobenzidine was used as the final chromogen.  Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Application	WB, IHC-P, IP, ELISA
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
IHC-P	1:200 - 1:1000
IP	1:100
ELISA	1:5000 - 1:20000

Not tested in other applications.

Calculated MW	128 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	85 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Purification	Antiserum From polyclonal serum
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	RB transcriptional corepressor like 2 , P130 , Rb2
Cellular Localization	Nucleus
Background	The product of the Retinoblastoma related human gene RB2 (p130) is highly homologous to the product of the Rb tumor suppressor gene, Rb (p105), especially in the functional pocket region of the protein. Certain carcinoma derived cell lines show low levels of Rbs expression, possibly due to gene rearrangement. In other experiments, a drastic reduction in proliferation is observed upon introduction of active Rb2.
Database	・ Gene ID: 5934 RBL2 ・ UniProt: Q08999 RBL2

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