抗Clathrin heavy chain [X22]抗体 | Anti-Clathrin heavy chain [X22] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of human colon tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:100
	ICC/IF analysis of HeLa cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:200 overnight at 4ºC
	WB analysis of 25 ug of human brain lysates using GTX22731 Clathrin heavy chain antibody [X22]. Dilution : 1:300
	ICC/IF analysis of NCI-H460 Cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:200 overnight at 4ºC
	ICC/IF analysis of U251 cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:200 overnight at 4ºC
	IHC-P analysis of human breast carcinoma tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:100

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	X22
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC, IM, Neutralizing/Blocking
Reactivity	Human, Mouse, Rat, Bovine, Dog, Hamster, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:100-1:500
ICC/IF	1:1,000
IHC-P	1:100
IHC-Fr	Assay dependent
FACS	1/200
IP	Assay dependent
IHC	Assay dependent
IM	Assay dependent
Neutralizing/Blocking	Assay dependent

Not tested in other applications.

Calculated MW	192 kDa. ( Note )
Specificity/Sensitivity	Electron microscopy and proteolysis mapping demonstrate that MA1-065 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	6 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Purified human brain clathrin heavy chain.
Purification	Protein A purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	clathrin heavy chain , CHC , CHC17 , CLH-17 , CLTCL2 , Hc , MRD56
Background	Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 1213 CLTC ・ UniProt: Q00610 CLTC

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Chao PK et al. Sci Rep 2019; 9 (1) : 2405 Convallatoxin enhance the ligand-induced mu-opioid receptor endocytosis and attenuate * antinociceptive tolerance in mice.	Application : WB Reactivity : Mouse
Chao PK et al. bioRxiv 2017; (Epub) Drug discovery to counteract antinociceptive tolerance with mu-opioid receptor endocytosis.	Application : IHC-Fr Reactivity : Mouse
Chao PK et al. bioRxiv 2017; (Epub) Drug discovery to counteract antinociceptive tolerance with mu-opioid receptor endocytosis.	Application : WB Reactivity : Mouse
Smith TH et al. J Biol Chem 2016; (Epub) Protease-activated Receptor-4 Signaling and Trafficking Is Regulated by the Clathrin Adaptor Protein Complex-2 Independent of β-arrestins.	Application : ICC/IF Reactivity : Human
Parks EE et al. J Biol Chem 2014; Cell Surface Epidermal Growth Factor Receptors Increase Src and c-Cbl Activity and Receptor Ubiquitylation.	Application : ICC/IF Reactivity : Human

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