抗PLK1 [13E8]抗体 | Anti-PLK1 [13E8] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of PLK1 knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15779 PLK1 antibody [13E8]. Dilution : 1 μg/ml
	ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Progression of cells through subsequent phases of the cell cycle results in differential localization of PLK1 in spindle poles, kinetochores and cleavage furrow.Green : Primary antibodyBlue : NucleiRed : ActinFixation : 4% paraformaldehydePermeabilization : 0.1% Triton X-100 for 10 minuteDilution : 3 μg/ml in 0.1% BSA and incubated for overnight at 4ºC
	ICC/IF analysis of WiDr cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC
	ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Fixation : 4% paraformaldehydePermeabilization : 0.1% Triton X-100 for 10 minuteDilution : 3 μg/ml in 0.1% BSA and incubated for overnight at 4ºC
	WB analysis of untreated or Nocodazole (100nM, 48 hours) treated U2OS lysate from non-targeting control or PLK1 siRNA transfected U2OS cells using GTX15779 PLK1 antibody [13E8]. Dilution : 1:1000 Loading : 25 μg
	IHC-P analysis of human colon tissue using GTX15779 PLK1 antibody [13E8]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:200
	ICC/IF analysis of U251 cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC
	WB analysis of whole cell extracts (30μg) of HeLa (Lane 1), treated with Nocodazole (500nM for 18 hrs) (Lane2), SW-480 (Lane 3), treated with Nocodazole (500nM for 18 hrs) (Lane 4), COLO 205 (Lane 5), HCT 116 (Lane 6), K-562 (Lane 7), MDA-MB-231 (Lane 8), HepG2 (Lane 9), A549 (Lane 10) using GTX15779 PLK1 antibody [13E8]. Dilution : 1:1000
	ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	13E8
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1,000
ICC/IF	3 μg/ml
IHC-P	1:20
IP	Assay dependent
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW	68 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant protein containing residues 300-400 of human PLK1.
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	polo like kinase 1 , PLK , STPK13
Cellular Localization	Nucleus
Background	The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
Database	・ Gene ID: 5347 PLK1 ・ UniProt: P53350 PLK1

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