TNF-alpha 組換え体(リコンビナント)タンパク質 | Recombinant Mouse TNF-alpha

掲載日情報:2015/01/26 現在Webページ番号:112683

R&D Systems社製の高品質なTNF-alphaの組換え体タンパク質(Recombinant Mouse TNF-alpha)です。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2024年03月30日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
TNF-α, Mouse, Recombinant
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 272
説明文
産生:E. coli,純度:>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.,由来動物:Mouse,M.W.:17 kDa,Genbank:7124,エンドトキシンレベル:<0.01 EU per 1 µg of the protein by the LAL method.
別名:APC1 protein
Genbank No: 7124
Protein Accession No: P06804
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 組換え体TNF-α
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
関連記事
TNF-α, Mouse, Recombinant, Carrier-free
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
産生:E. coli,純度:>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.,由来動物:Mouse,M.W.:17 kDa,Genbank:7124,エンドトキシンレベル:<0.01 EU per 1 µg of the protein by the LAL method.
別名:APC1 protein
Genbank No: 7124
Protein Accession No: P06804
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 組換え体TNF-α
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
関連記事

[在庫・価格 :2024年03月30日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

TNF-α, Mouse, Recombinant

文献数: 272

説明文 産生:E. coli,純度:>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.,由来動物:Mouse,M.W.:17 kDa,Genbank:7124,エンドトキシンレベル:<0.01 EU per 1 µg of the protein by the LAL method.
別名:APC1 protein
Genbank No: 7124
Protein Accession No: P06804
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 組換え体TNF-α
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
関連記事

TNF-α, Mouse, Recombinant, Carrier-free

文献数: 0

説明文 産生:E. coli,純度:>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.,由来動物:Mouse,M.W.:17 kDa,Genbank:7124,エンドトキシンレベル:<0.01 EU per 1 µg of the protein by the LAL method.
別名:APC1 protein
Genbank No: 7124
Protein Accession No: P06804
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 組換え体TNF-α
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
関連記事

バルク注文に関して

バルク包装品も承ります。お気軽にお問合せ下さい。

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CF(Carrier-Free)とは?

R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。

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Product Details

Source
E. coli-derived, Leu80-Leu235, with an N-terminal Met
Accession
P06804
Structure
A small amount of recombinant protein lacking the N-terminal methionine and four additional amino acid residues may be also present.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Predicted Molecular Mass
17 kDa
Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds)

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Additional Information

Molecule Information
TNF-alpha
Aliases
Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Background
TNF-alpha Background
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

A pseudopterane diterpene isolated from the octocoral Pseudopterogorgia acerosa inhibits the inflammatory response mediated by TLR-ligands and TNF-alpha in macrophages.
Barros de Arruda L, Doens D, Fernandez P, Gonzalez Y, Gutierrez M, Lleonart R, Ramos M, Restrepo C, Santamaria R
PLoS ONE 2013 8:e84107
Species: Mouse
Protein Application: cell culture


Host-derived MCP-1 and MIP-1alpha regulate protective anti-tumor immunity to localized and metastatic B16 melanoma.
Fujimoto M, Hamaguchi Y, Hasegawa M, Huu DL, Matsushita T, Nakasone Y, Sato S, Takehara K, Yanaba M
Am. J. Pathol. 2012 180:365-74
Species: Mouse
Protein Application: In Vivo


Matrix metalloprotease 9 mediates neutrophil migration into the airways in response to influenza virus-induced toll-like receptor signaling.
Akira S, Baaten BJ, Bradley LM, Chatterjee D, Douglass MF
PLoS Pathog. 2012 8:e1002641
Species: Mouse
Protein Application: Bioassay


Hepatocyte-specific mutation of both NF-kappaB RelA and STAT3 abrogates the acute phase response in mice.
Akira S, Algul H, Allen E, Blahna MT, Ferrari JD, Hilliard KL, Jones MR, Mizgerd JP, Pelton SI, Quinton LJ, Sabharwal V, Schmid RM, Spira A, Zhang X
J. Clin. Invest. 2012 122:1758-63
Species: Mouse
Protein Application: In Vivo


Hepatocarcinogenesis in FXR-/- mice mimics human HCC progression that operates through HNF1alpha regulation of FXR expression.
Forman BM, Huang W, Lai L, Liu N, Liu X, Lou G, Meng Z, Wang X, Xu R, Xu Z, Yen Y, Zhang L, Zhang Y, Zhou W
Mol. Endocrinol. 2012 26:775-85
Species: Mouse
Protein Application: Bioassay


The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
Chuquimia OD, Fernandez C, Hartl K, Petursdottir DH, Rahman MJ, Singh M
PLoS ONE 2012 7:e32125
Species: Mouse
Protein Application: Bioassay


Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
D'Acquisto F, Jones GE, Nourshargh S, Proebstl D, Rowe D, Voisin MB, Whiteford J, Woodfin A
J. Exp. Med. 2012 209:1219-34
Species: Mouse
Protein Application: In Vivo


Eukaryotic initiation factor 2 (eIF2) signaling regulates proinflammatory cytokine expression and bacterial invasion.
Bahnan W, Barber G, Fields K, Schesser K, Shrestha N, Wiley D
J Biol Chem 2012 287:28738-44

Regulation of innate CD8+ T-cell activation mediated by cytokines.
Proc Natl Acad Sci U S A 2012 109:9971-6

IL-17A and TNF-alpha Exert Synergistic Effects on Expression of CXCL5 by Alveolar Type II Cells In Vivo and In Vitro.
Dai N, Favara M, Gonzales L, Guttentag S, Liu Y, Malcolm KC, Mei J, Wang P, Worthen GS, Yang G, Zhang P
J. Immunol. 2011 186:3197-205
Species: Mouse
Protein Application: In Vivo


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