Rat VEGF Quantikine ELISA Kit | rVEGF Qkit
掲載日情報：2016/08/18 現在Webページ番号：111401
R&D Systems製のRat VEGFを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 15時15分現在]
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VEGF, Rat, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2025年04月26日 15時15分現在]
VEGF, Rat, ELISA Kit, Quantikine (96 well)
文献数： 73
- 商品コード：RRV00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：31.2-2,000 pg/mL，感度：25 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Rat，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：3.5 hours，測定因子：VEGF 別名：MVCD1 Genbank No： 7422 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2019年12月15日号 p.3
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| 製品記事 | R&D Systems社 VEGF-VEGF R2シグナル伝達系関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 25 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant rat VEGF
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Rat VEGF Immunoassay is a 3.5 hour solid-phase ELISA designed to measure rat VEGF in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat VEGF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat VEGF.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 65 | 60 | 65 |
| Mean | 79.4 | 174 | 797 | 87.6 | 204 | 828 |
| Standard Deviation | 5.7 | 7 | 14.1 | 8.2 | 21.2 | 42.5 |
| CV% | 7.2 | 4 | 1.8 | 9.4 | 10.4 | 5.1 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 71 | 65 | 71 |
| Mean | 103 | 191 | 891 | 107 | 233 | 900 |
| Standard Deviation | 3.8 | 10.7 | 19.7 | 8.5 | 23.3 | 41.1 |
| CV% | 3.7 | 5.6 | 2.2 | 7.9 | 10 | 4.6 |
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Recovery
The recovery of rat VEGF spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 99 | 90-116 |
| Heparin Plasma (n=6) | 92 | 80-100 |
| Cell Culture Supernates (n=6) | 103 | 94-108 |
| EDTA Plasma (n=6) | 102 | 95-108 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of rat VEGF in each matrix were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- VEGF
- Aliases
- VAS; Vasculotropin; VEGFA; VPF
- Entrez Gene IDs
- 7422 (Human); 22339 (Mouse); 83785 (Rat); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
- Background
- VEGF
- Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 - 42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. The amino acid sequence of VEGF exhibits primary structural, as well as limited amino acid sequence, homology with that of the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfide bonds are conserved among these growth factors. A cDNA encoding a protein having a 53% amino acid sequence homology in the PDGF-like region of VEGF has been isolated from a human placental cDNA library. This protein, named placenta growth factor (PlGF), is now recognized to be a member of the VEGF family of growth factors. Based on its homology with VEGF, PlGF was also proposed to be an angiogenic factor. Two receptor tyrosine kinases have been described as putative VEGF receptors. Flt-1 (fms-like tyrosine kinase), and KDR (kinase-insert-domain-containing receptor) proteins have been shown to bind VEGF with high affinity.In vitro, VEGF is a potent endothelial cell mitogen. In cultured endothelial cells, VEGF can activate phospholipase C and induce rapid increases of free cytosolic Ca2+. VEGF has been shown to stimulate von Willebrand factor release from endothelial cells and induce expression of tissue factor activity in endothelial cells as well as in monocytes. VEGF has also been shown to be chemotactic for monocytes and osteoblasts. In vivo, VEGF can induce angiogenesis as well as increase microvascular permeability. As a vascular permeability factor, VEGF acts directly on the endothelium and does not degranulate mast cells. It promotes extravasation of plasma fibrinogen, leading to fibrin deposition which alters the tumor extracellular matrix. The modified extracellular matrix subsequently promotes the migration of macrophages, fibroblasts and endothelial cells. Based on its in vitro and in vivo properties, VEGF is expected to play important roles in inflammation and during normal and pathological angiogenesis, a process that is associated with wound healing, embryonic development, and growth and metastasis of solid tumors. Elevated levels of VEGF have been reported in synovial fluids of rheumatoid arthritis patients and in sera from cancer patients.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function.
Aida , Takuya, Aono , Fumihito, Aoyama , Naoki, Ezoe , Kenji, Kato , Keiich, Kato , Osamu, Kuroda , Tomoko, Murata , Nana, Nakama , Ken, Sano , Chiaki, Takehara , Yuji, Yabuuchi , Akiko, Yamadera , Rie
Lab Invest 2013 93:181-93
Species:
Sample Type: Cell Culture Supernates
Angiopoietins/TIE2 system and VEGF are involved in ovarian function in a DHEA rat model of polycystic ovary syndrome.
Abramovich D, Bas D, Cataldi NI, Irusta G, Parborell F, Tesone M
Endocrinology 2012 153:3446-56
Species: Rat
Sample Type: Tissue Homogenates
Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
Deng J, Han Y, Kang J, Li S, Tao J, Tian X, Yan C
Apoptosis 2010 15:463-73
Species: Rat
Sample Type: Cell Culture Supernates
Toll-like receptor 2 mediates mesenchymal stem cell-associated myocardial recovery and VEGF production following acute ischemia-reperfusion injury.
Abarbanell AM, Herrmann JL, Wang Y
Am. J. Physiol. Heart Circ. Physiol. 2010 298:H1529-36
Species: Mouse
Sample Type: Cell Culture Supernates
Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1.
Aragones J, Belin MF, Carmeliet P, Chedotal A, Chounlamountri N, Coulon C, Dassonville K, Dhondt J, D'Hooge R, Dresselaers T, Eriksson U, Gall D, Gallez B, Geudens I, Haigh JJ, Hermans K, Honnorat J, Jordan BF, Knevels E, Lambrechts D, Meissirel C, Moons L, Mutin M, Poesen K, Renaud J, Ruiz de Almodovar C, Salin PA, Schiffmann S, Storkebaum E, Stroobants S, Thomasset N, Van Geyte K, Van Hecke P, Vinckier S, Zacchigna S
J. Neurosci. 2010 30:15052-66
Species: Rat
Sample Type: Tissue Homogenates
Angiogenesis is present in experimental autoimmune encephalomyelitis and pro-angiogenic factors are increased in multiple sclerosis lesions.
Brinkmann V, Hiestand PC, Littlewood-Evans A, Pollinger B, Schnell C, Seabrook TJ
J Neuroinflammation 2010 7:95
Species: Rat
Sample Type: Tissue Homogenates
Phosphomannopentaose sulfate (PI-88) inhibits retinal leukostasis in diabetic rat.
Hu J, Luo Y, Ma H, Ma P, Tang S, Zhu X
Biochem. Biophys. Res. Commun. 2009 380:402-6
Species: Rat
Sample Type: Tissue Homogenates
The role of supplemental oxygen and JAK/STAT signaling in intravitreous neovascularization in a ROP rat model.
Budd S, Byfield G, Hartnett ME
Invest. Ophthalmol. Vis. Sci. 2009 50:3360-5
Species: Rat
Sample Type: Tissue Homogenates
Poly(Adenosine 5'-diphosphate-ribose) polymerase inhibition counteracts multiple manifestations of experimental type 1 diabetic nephropathy.
Drel VR, Obrosova IG, Pavlov IA, Shevalye H, Slusher B, Xu W, Zhang J
Endocrinology 2009 150:5273-83
Species: Rat
Sample Type: Tissue Homogenates
The effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine.
Ashley SW, Krebs NJ, Leach JK, Mooney DJ, Rocha FG, Sundback CA, Vacanti JP, Whang EE
Biomaterials 2008 29:2884-90
Species: Rat
Sample Type: Tissue Homogenates
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