Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit | rIL-1a Qkit

• Product Details
• Product Summary
• Precision
• Recovery
• Linearity
• Additional Infomation
• Citations

Product Details

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL)

Sensitivity

4.12 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)

Specificity

Natural and recombinant rat IL-1 alpha

Interference

Interference observed with 1 or more available related molecules.

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Product Summary

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Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	54	134	417	54	130	449
Standard Deviation	3.6	6.3	16.5	5	9.5	29.7
CV%	6.7	4.7	4	9.3	7.3	6.6

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Recovery

Sample Type	Average % Recovery	Range %
Serum (n=4)	92	85-99
Cell Culture Supernates (n=4)	104	100-111
EDTA Plasma (n=5)	98	89-112

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Linearity

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Additional Infomation

Molecule Information

IL-1 alpha/IL-1F1

Aliases

IL1A; IL-1F1

Entrez Gene IDs

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)

Background

IL-1 alpha/IL-1F1

Interleukin-1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL 4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Recent evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

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Citations

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