Rat Total MMP-9 Quantikine ELISA Kit | rTot MMP-9 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111398
R&D Systems製のRat Total MMP-9を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月03日 20時55分現在]
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MMP-9, Total, Rat, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月03日 20時55分現在]
MMP-9, Total, Rat, ELISA Kit, Quantikine (96well)
文献数： 10
- 商品コード：RMP900
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：0.028 ng/mL，測定範囲：0.16 - 10 ng/mL，種交差性：Rat，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor Heparin Plasma (10 uL)，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：MMP-9 別名：92 kDa gelatinase Genbank No： 4318 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Recombinant Human SCGF-alpha |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor Heparin Plasma (10 µL)
- Sensitivity
- 0.028 ng/mL
- Assay Range
- 0.156 - 10 ng/mL
- Specificity
- Natural and recombinant rat MMP-9 (Pro-, active, and TIMP-complexed)
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Rat Total MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total rat MMP-9 (Pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat MMP-9 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat MMP-9.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.39 | 1.22 | 5.27 | 0.51 | 1.18 | 3.55 |
| Standard Deviation | 0.027 | 0.068 | 0.238 | 0.035 | 0.046 | 0.153 |
| CV% | 6.9 | 5.6 | 4.5 | 6.9 | 3.9 | 4.3 |
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Recovery
The recovery of rat MMP-9 spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=4) | 94 | 87-107 |
| Serum (n=4) | 97 | 90-115 |
| Platelet-poor Heparin Plasma (n=4) | 92 | 80-103 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of rat MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- MMP-9
- Aliases
- CLG4B; Gelatinase B; GELB
- Entrez Gene IDs
- 4318 (Human); 17395 (Mouse); 81687 (Rat)
- Background
- Matrix Metalloproteinases (MMPs)
- The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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