"Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate" | rIL-1b Qkit

掲載日情報:2016/08/18 現在Webページ番号:111397

R&D Systems製の"Rat IL-1 beta/IL-1F2, 2 Plate"を測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2022年08月12日 13時51分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
IL-1β, Rat, ELISA Kit, Quantikine M (2×96 well)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 175
説明文
測定範囲:31.2-2,000 pg/mL ,感度:5 pg/mL,測定試料:培養上清, 血清, 血漿(EDTA),種交差性:Rat,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:2×96 well,測定時間:4.5 hours,測定因子:IL-1 beta/IL-1F2
別名:catabolin
Genbank No: 3553
別包装品 別包装品あり
法規制等 医薬用外毒物
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

[在庫・価格 :2022年08月12日 13時51分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

IL-1β, Rat, ELISA Kit, Quantikine M (2×96 well)

文献数: 175

説明文 測定範囲:31.2-2,000 pg/mL ,感度:5 pg/mL,測定試料:培養上清, 血清, 血漿(EDTA),種交差性:Rat,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:2×96 well,測定時間:4.5 hours,測定因子:IL-1 beta/IL-1F2
別名:catabolin
Genbank No: 3553
別包装品 別包装品あり
法規制等 医薬用外毒物
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (17 µL), EDTA Plasma (17 µL)

Sensitivity
5 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
Specificity
Natural and recombinant rat IL-1 beta
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Rat IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 beta in cell culture supernates, serum, and EDTA plasma. It contains recombinant rat IL-1 beta and antibodies raised against recombinant rat IL-1 beta. This immunoassay has been shown to quantitate the recombinant rat factor accurately.

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Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 83.4 253 610 82.3 249 626
Standard Deviation 7.3 9.9 24 4.7 10.3 27
CV% 8.8 3.9 3.9 5.7 4.1 4.4

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Recovery

The recovery of rat IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=5) 108 97-120
Cell Culture Supernates (n=5) 100 89-110
EDTA Plasma (n=5) 98 89-111

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Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of rat IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.

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Additional Infomation

Molecule Information
IL-1 beta/IL-1F2
Aliases
IL1B; IL-1F2
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 100034237 (Equine); 100135556 (Guinea Pig)
Background
IL-1 beta/IL-1F2
IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways, with no additional protection from prolonged infusion.
Cytokine 2013 61:614-21
Species: Rat
Sample Type: BALF


Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
J Neuroinflammation 2012 9:181

Evidence that intrathecal morphine-3-glucuronide may cause pain enhancement via toll-like receptor 4/MD-2 and interleukin-1beta.
Hutchinson MR, Lewis SS, Loram LC, Maier SF, Rezvani N, Rice KC, Watkins LR, Zhang Y
Neuroscience 2010 165:569-83
Species: Rat
Sample Type: CSF


Neuregulin-ErbB signaling promotes microglial proliferation and chemotaxis contributing to microgliosis and pain after peripheral nerve injury.
Bennett DL, Calvo M, Grist J, Loeb JA, Ma Z, Tsantoulas C, Zhu N
J. Neurosci. 2010 30:5437-50
Species: Rat
Sample Type: Cell Culture Supernates


House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
Belvisi MG, Birrell MA, Collins M, De Alba J, Dekkak A, Knowles RG, Nials AT, Raemdonck K, Wong S
Eur. Respir. J. 2010 35:1377-87
Species: Rat
Sample Type: BALF


Identification of a serum-induced transcriptional signature associated with type 1 diabetes in the BioBreeding rat.
Basken J, Geoffrey R, Hessner MJ, Jia S, Kaldunski M, Kansra S, Lernmark A, Mordes JP, Prosser S, Wang X
Diabetes 2010 59:2375-85
Species: Rat
Sample Type: Cell Culture Supernates


Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
Lim G, Mao J, Tian Y, Wang S, Zhang Y
J. Clin. Invest. 2009 119:295-304
Species: Rat
Sample Type: Cell Culture Supernates


Role of sensory innervation in the rat pulmonary neutrophil recruitment induced by staphylococcal enterotoxins type A and B.
Antunes E, Camargo EA, Costa SK, De Nucci G, Desouza IA, Mariano NS, Mello GC, Optiz-Neto JB, Resende JS
Eur. J. Pharmacol. 2009 613:128-34
Species: Rat
Sample Type: BALF


Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
Hu S, Summy-Long JY
Am. J. Physiol. Regul. Integr. Comp. Physiol. 2009 297:R1532-45
Species: Rat
Sample Type: Plasma


In vivo Angiotensin II AT1 receptor blockade selectively inhibits LPS-induced innate immune response and ACTH release in rat pituitary gland.
Benicky J, Pavel J, Saavedra JM, Sanchez-Lemus E
Brain Behav. Immun. 2009 23:945-57
Species: Rat
Sample Type: Tissue Homogenates


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