"Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit" | rIL-1b Qkit
掲載日情報：2016/08/18 現在Webページ番号：111397
R&D Systems製の"Rat IL-1 beta/IL-1F2"を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月24日 08時55分現在]
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IL-1β, Rat, ELISA Kit, Quantikine M (1Plate) |
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[在庫・価格 ：2024年04月24日 08時55分現在]
IL-1β, Rat, ELISA Kit, Quantikine M (1Plate)
文献数： 175
- 商品コード：RLB00-1
- メーカー：RSD
- 包装：1kit
- 価格：¥104,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：31.2-2,000 pg/mL ，感度：5 pg/mL，測定試料：培養上清, 血清, 血漿(EDTA)，種交差性：Rat，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-1 beta/IL-1F2 別名：catabolin Genbank No： 3553 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
R&D Systems社 IL-1β関連製品 |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 R&D Systems マウス／ラット用Quantikine ELISA Kit 2 plate製品の包装変更について |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (17 µL), EDTA Plasma (17 µL)
- Sensitivity
- 5 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant rat IL-1 beta
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Rat IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 beta in cell culture supernates, serum, and EDTA plasma. It contains recombinant rat IL-1 beta and antibodies raised against recombinant rat IL-1 beta. This immunoassay has been shown to quantitate the recombinant rat factor accurately.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 83.4 | 253 | 610 | 82.3 | 249 | 626 |
| Standard Deviation | 7.3 | 9.9 | 24 | 4.7 | 10.3 | 27 |
| CV% | 8.8 | 3.9 | 3.9 | 5.7 | 4.1 | 4.4 |
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Recovery
The recovery of rat IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 108 | 97-120 |
| Cell Culture Supernates (n=5) | 100 | 89-110 |
| EDTA Plasma (n=5) | 98 | 89-111 |
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Linearity
To assess the linearity of the assay, five or more samples spiked with various concentrations of rat IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-1 beta/IL-1F2
- Aliases
- IL1B; IL-1F2
- Entrez Gene IDs
- 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 100034237 (Equine); 100135556 (Guinea Pig)
- Background
- IL-1 beta/IL-1F2
- IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways, with no additional protection from prolonged infusion.
Cytokine 2013 61:614-21
Species: Rat
Sample Type: BALF
Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
J Neuroinflammation 2012 9:181
Evidence that intrathecal morphine-3-glucuronide may cause pain enhancement via toll-like receptor 4/MD-2 and interleukin-1beta.
Hutchinson MR, Lewis SS, Loram LC, Maier SF, Rezvani N, Rice KC, Watkins LR, Zhang Y
Neuroscience 2010 165:569-83
Species: Rat
Sample Type: CSF
Neuregulin-ErbB signaling promotes microglial proliferation and chemotaxis contributing to microgliosis and pain after peripheral nerve injury.
Bennett DL, Calvo M, Grist J, Loeb JA, Ma Z, Tsantoulas C, Zhu N
J. Neurosci. 2010 30:5437-50
Species: Rat
Sample Type: Cell Culture Supernates
House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
Belvisi MG, Birrell MA, Collins M, De Alba J, Dekkak A, Knowles RG, Nials AT, Raemdonck K, Wong S
Eur. Respir. J. 2010 35:1377-87
Species: Rat
Sample Type: BALF
Identification of a serum-induced transcriptional signature associated with type 1 diabetes in the BioBreeding rat.
Basken J, Geoffrey R, Hessner MJ, Jia S, Kaldunski M, Kansra S, Lernmark A, Mordes JP, Prosser S, Wang X
Diabetes 2010 59:2375-85
Species: Rat
Sample Type: Cell Culture Supernates
Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
Lim G, Mao J, Tian Y, Wang S, Zhang Y
J. Clin. Invest. 2009 119:295-304
Species: Rat
Sample Type: Cell Culture Supernates
Role of sensory innervation in the rat pulmonary neutrophil recruitment induced by staphylococcal enterotoxins type A and B.
Antunes E, Camargo EA, Costa SK, De Nucci G, Desouza IA, Mariano NS, Mello GC, Optiz-Neto JB, Resende JS
Eur. J. Pharmacol. 2009 613:128-34
Species: Rat
Sample Type: BALF
Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
Hu S, Summy-Long JY
Am. J. Physiol. Regul. Integr. Comp. Physiol. 2009 297:R1532-45
Species: Rat
Sample Type: Plasma
In vivo Angiotensin II AT1 receptor blockade selectively inhibits LPS-induced innate immune response and ACTH release in rat pituitary gland.
Benicky J, Pavel J, Saavedra JM, Sanchez-Lemus E
Brain Behav. Immun. 2009 23:945-57
Species: Rat
Sample Type: Tissue Homogenates
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