Rat ICAM-1/CD54 Quantikine ELISA Kit | rICAM-1/CD54 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111394
R&D Systems製のRat ICAM-1/CD54を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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ICAM-1, Soluble, Rat, ELISA Kit, Quantikine M (96 well) <CD54, ELISA Kit> |
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[在庫・価格 ：2025年04月27日 00時00分現在]
ICAM-1, Soluble, Rat, ELISA Kit, Quantikine M (96 well) <CD54, ELISA Kit>
文献数： 22
- 商品コード：RIC100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：31.2-2,000 pg/mL，感度：4.1 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Rat，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：ICAM-1, CD54 別名：BB2 Genbank No： 3383 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | R&D Systems 社 sICAM-1/CD54関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
- Sensitivity
- 4.1 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant rat sICAM-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Rat sICAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat sICAM-1 levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant rat sICAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring rat sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural rat sICAM-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 47 | 39 | 47 |
| Mean | 87 | 271 | 617 | 80 | 307 | 560 |
| Standard Deviation | 4.3 | 13 | 29 | 7.6 | 19 | 35 |
| CV% | 4.9 | 4.8 | 4.7 | 9.5 | 6.2 | 6.2 |
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Recovery
The recovery of rat sICAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 103 | 83-119 |
| Cell Culture Supernates (n=8) | 99 | 88-104 |
| EDTA Plasma (n=5) | 103 | 91-117 |
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Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of rat sICAM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- ICAM-1/CD54
- Aliases
- CD54
- Entrez Gene IDs
- 3383 (Human); 15894 (Mouse); 25464 (Rat)
- Background
- ICAM-1/CD54
- Intercellular adhesion molecule 1 (ICAM-1), also known as CD54, binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats.
Andersson G, Hu L, Jacobson A, Lind PM, Lind T, Melhus H, Risteli J, Rodriguez-Navarro A, Sundqvist A, Yebra-Rodriguez A
Bone 2011 48:496-506
Species: Rat
Sample Type: Serum
Nuclear factor-kappaB activation by edema inhibits intestinal contractile activity.
Cox CS, Kislitsyna K, Uray KS, Wright Z, Xue H
Crit. Care Med. 2010 38:861-70
Species: Rat
Sample Type: Cell Lysates
Poly(ADP-ribose) polymerase (PARP) inhibition counteracts multiple manifestations of kidney disease in long-term streptozotocin-diabetic rat model.
Drel VR, Floyd EZ, Lupachyk S, Maksimchyk Y, Obrosova IG, Shevalye H, Slusher B, Stavniichuk R, Xu W, Zhang J
Biochem. Pharmacol. 2010 79:1007-14
Species: Rat
Sample Type: Tissue Homogenates
Hyperglycemia enhances excessive superoxide anion radical generation, oxidative stress, early inflammation, and endothelial injury in forebrain ischemia/reperfusion rats.
Fujita M, Kasaoka S, Koda Y, Koga Y, Maekawa T, Maruyama I, Nanba M, Ono T, Shitara M, Tsuruta R, Yamamoto T, Yuasa M
Brain Res. 2010 1309:155-63
Species:
Sample Type: Tissue Homogenates
Cholinergic agonist physostigmine suppresses excessive superoxide anion radical generation in blood, oxidative stress, early inflammation, and endothelial injury in rats with forebrain ischemia/reperfusion.
Fujita M, Igarashi M, Izumi T, Kasaoka S, Kutsuna S, Maekawa T, Ogino Y, Takahashi K, Todani M, Tsuruta R, Yagi T, Yuasa M
Brain Res. 2010 1313:242-9
Species: Rat
Sample Type: Tissue Homogenates
Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage.
Almulki L, Arita R, Hafezi-Moghadam A, Hata Y, Ishibashi T, Kawahara S, Kita T, Miura M, Nakao S, Shimokawa H, Tayyari F, Zandi S
Diabetes 2009 58:215-26
Species: Rat
Sample Type: Cell Lysates
Elevation of jugular venous superoxide anion radical is associated with early inflammation, oxidative stress, and endothelial injury in forebrain ischemia-reperfusion rats.
Aki HS, Aoki T, Fujimoto K, Fujita M, Kasaoka S, Kumagai K, Maekawa T, Maruyama I, Murata H, Nanba M, Tsuruta R, Yamashita S, Yuasa M
Brain Res. 2009 1292:180-90
Species: Rat
Sample Type: Tissue Homogenates
Maternal diet influences DNA damage, aortic telomere length, oxidative stress, and antioxidant defense capacity in rats.
Chen JH, Cripps RL, Martin-Gronert MS, Ozanne SE, Tarry-Adkins JL
FASEB J. 2008 22:2037-44
Species: Rat
Sample Type: Serum
Vascular adhesion protein-1 blockade suppresses choroidal neovascularization.
Almulki L, Garland RC, Gragoudas ES, Hafezi-Moghadam A, Hisatomi T, Ito Y, Mashima Y, Miller JW, Miyahara S, Nakao S, Nakazawa T, Noda K, She H, Thomas KL, Zandi S
FASEB J. 2008 22:2928-35
Species: Rat
Sample Type: Tissue Homogenates
Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway.
Bland KI, Chaudry IH, Choudhry MA, Hsieh YC, Schwacha MG, Suzuki T, Yu HP
J. Leukoc. Biol. 2007 82:774-80
Species: Rat
Sample Type: Tissue Homogenates
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