Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit | m/rFABP1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111392
R&D Systems製のMouse/Rat FABP1/L-FABPを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月28日 00時00分現在]
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[在庫・価格 ：2024年04月28日 00時00分現在]
Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit
文献数： 4
- 商品コード：RFBP10
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：0.029 ng/mL，測定範囲：0.08 - 5 ng/mL，種交差性：Mouse/Rat，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：FABP1/L-FABP 別名：FABPL Genbank No： 2168 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Mouse / Rat FABP1 ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
- Sensitivity
- 0.029 ng/mL
- Assay Range
- 0.078 - 5 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant FABP1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse/Rat FABP1 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FABP1 in mouse or rat cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant rat FABP1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant rat FABP1. Results obtained using natural mouse or rat FABP1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural FABP1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.28 | 0.69 | 1.22 | 0.27 | 0.68 | 1.25 |
| Standard Deviation | 0.01 | 0.02 | 0.03 | 0.01 | 0.03 | 0.06 |
| CV% | 3.6 | 2.9 | 2.5 | 3.7 | 4.4 | 4.8 |
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Recovery
The recovery of FABP1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 95 | 90-100 |
| Rat Serum (n=4) | 100 | 87-116 |
| Rat EDTA Plasma (n=4) | 94 | 84-107 |
| Rat Heparin Plasma (n=4) | 100 | 92-111 |
| Mouse Urine (n=4) | 96 | 86-108 |
| Rat Urine (n=4) | 99 | 95-105 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FABP1 in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- FABP1/L-FABP
- Aliases
- L-FABP
- Entrez Gene IDs
- 2168 (Human); 14080 (Mouse); 24360 (Rat)
- Background
- FABP1
- Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives. FABP1 is thought to be involved in intracellular lipid transport.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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