Human IL-6 QuantiGlo ELISA Kit
掲載日情報：2016/08/18 現在Webページ番号：111379
R&D Systems製のHuman IL-6を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月02日 11時35分現在]
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IL-6, Human, ELISA Kit, QuantiGlo, 2nd Generation (96 well) |
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[在庫・価格 ：2024年05月02日 11時35分現在]
IL-6, Human, ELISA Kit, QuantiGlo, 2nd Generation (96 well)
文献数： 33
- 商品コード：Q6000B
- メーカー：RSD
- 包装：1kit
- 価格：¥135,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.48 - 1500 pg/mL，感度：0.35 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)，種交差性：Human，検出方法：化学発光，アッセイ法：サンドイッチELISA，アッセイ数：96 well，別売品コントロール：#QC193，測定時間：5.5 hours，測定因子：IL-6 別名：B cell stimulatory factor-2 Genbank No： 3569 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 |
QuantiGlo Chemiluminescent Sandwich ELISA Kit 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 5.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
- Sensitivity
- 0.35 pg/mL
- Assay Range
- 0.48 - 1,500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-6
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The QuantiGlo Human IL-6 Chemiluminescent Immunoassay is a solid phase ELISA that specifically measures human IL-6 in less than 5.5 hours. This kit is designed to measure IL-6 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-6 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6. It has been observed in our laboratories that the measurement of human IL-6 is insensitive to the addition of the recombinant form of the human IL-6 soluble receptor.
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Precision
Intra-Assay Precision (Precision within an assay)
Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||||
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.21 | 10.7 | 118 | 766 | 1.56 | 13.2 | 142 | 752 |
| Standard Deviation | 0.07 | 0.32 | 4.15 | 37.5 | 0.15 | 1.02 | 8.93 | 53.7 |
| CV% | 5.8 | 3 | 3.5 | 4.9 | 9.6 | 7.7 | 6.3 | 7.1 |
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Recovery
The recovery of IL-6 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 103 | 98-113 |
| Serum (n=4) | 94 | 88-106 |
| EDTA Plasma (n=4) | 89 | 82-95 |
| Heparin Plasma (n=4) | 88 | 80-97 |
| Citrate Plasma (n=4) | 87 | 82-96 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-6 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-6
- Aliases
- BSF-2; IFN-beta 2; MGI-2A
- Entrez Gene IDs
- 3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
- Background
- IL-6
- Interleukin 6 (IL-6), also known as interferon-beta 2, 26 kDa protein and B cell stimulatory factor-2 (BSF-2), is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa - 28 kDa phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties.IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Inflammation, coagulation and cardiovascular disease in HIV-infected individuals.
Belloso , Waldo, De Wit , Stephane, Drummond , Fraser, Duprez , Daniel A, Kuller , Lewis H, Lane , H Cliffo, Ledergerber , Bruno, Lundgren , Jens, Neaton , James D, Neuhaus , Jacqueli, Nixon , Daniel, Paton , Nicholas, Prineas , Ronald J, Tracy , Russell
PLoS ONE 2012 7:e44454
Species: Human
Sample Type: Plasma
Angiotensin converting enzyme inhibitor and HMG-CoA reductase inhibitor as adjunct treatment for persons with HIV infection: a feasibility randomized trial.
Baker J, Duprez D, Grimm R, Henry K, Huppler Hullsiek K, Neaton J, Prosser R, Rhame F, Tracy R
PLoS ONE 2012 7:e46894
Species: Human
Sample Type: Plasma
Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue.
Acevedo I, Ben-Jonathan N, Cifuentes M, Fuentes C, Hugo E, Reyes M, Tobar N, Villalobos E
Mol Cell Endocrinol 2012 361:24-30
Early inflammatory and metabolic changes in association with AGTR1 polymorphisms in prehypertensive subjects.
Fung MM, Poddar S, Rao F
Am. J. Hypertens. 2011 24:225-33
Species: Human
Sample Type: Plasma
Activation of nuclear factor-kappa B signalling promotes cellular senescence.
Alvarez-Fernandez M, Caballero OL, Caetano C, Hummerich H, Jat PS, Mansfield L, Medema RH, Rovillain E
Oncogene 2011 30:2356-66
Species: Human
Sample Type: Cell Culture Supernates
Biomarkers of inflammation and development of rheumatoid arthritis in women from two prospective cohort studies.
Buring JE, Chibnik LB, Costenbader KH, Karlson EW, Lee IM, Manson JE, Shadick NA, Tworoger SS
Arthritis Rheum. 2009 60:641-52
Species: Human
Sample Type: Plasma
Obesity in pregnancy stimulates macrophage accumulation and inflammation in the placenta.
Basu S, Bintein T, Catalano PM, Challier JC, Hauguel-De Mouzon S, Hotmire K, Minium J
Placenta 2008 29:274-81
Species: Human
Sample Type: Plasma
Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Cummins JE, Curtis K, Dezzutti CS, Donaghay M, Doncel GF, Donoval B, Fichorova RN, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pallansch-Cokonis M, Pasicznyk JM, Poli G, Reichelderfer P, Richardson-Harman N, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R
Anal. Chem. 2008 80:4741-51
Species: Human
Species: N/A
Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes.
Figulla HR, Foerster M, Fritzenwanger M, Krack A, Kuethe F, Meusel K
Cytokine 2007 38:137-44
Species: Human
Sample Type: Cell Culture Supernates
Usefulness of C-reactive protein and interleukin-6 as predictors of outcomes in patients with chronic obstructive pulmonary disease receiving pravastatin.
Chang NC, Lee TM, Lin MS
Am. J. Cardiol. 2007 101:530-5
Species: Human
Sample Type: Plasma
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