Mouse LIX Quantikine ELISA Kit | mLIX Qkit
掲載日情報：2016/08/18 現在Webページ番号：111370
R&D Systems製のMouse LIXを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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Mouse CXCL5/LIX Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Mouse CXCL5/LIX Quantikine ELISA Kit
文献数： 7
- 商品コード：MX000
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 種交差性：Mouse，感度：1.74 pg/mL，測定範囲：15.6-1,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Tissue Homogenates，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：LIX Genbank No： 6374 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine Mouse CXCL5 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (13 µL), Platelet-poor Heparin Plasma (13 µL), Urine (13 µL)
- Sensitivity
- 1.74 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Tissue Homogenates, Urine)
- Specificity
- Natural and recombinant mouse LIX
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse CXCL5/LIX Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIX levels in cell culture supernates, tissue lysates, serum, platelet-poor plasma, and urine. It contains E. coli-expressed recombinant mouse LIX and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse LIX accurately. Results obtained using natural mouse LIX showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse LIX.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 42.7 | 133 | 407 | 41.8 | 120 | 393 |
| Standard Deviation | 1.72 | 5.93 | 10.9 | 2.81 | 4.64 | 20.3 |
| CV% | 4 | 4.5 | 2.7 | 6.7 | 3.9 | 5.2 |
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Recovery
The recovery of mouse LIX spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=4) | 114 | 105-120 |
| Tissue Homogenates (n=4) | 100 | 87-113 |
| Serum (n=4) | 87 | 82-94 |
| Platelet-poor EDTA Plasma (n=4) | 100 | 92-109 |
| Platelet-poor Heparin Plasma (n=4) | 91 | 85-99 |
| Urine (n=4) | 91 | 84-96 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse LIX were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- LIX
- Aliases
- LIX
- Entrez Gene IDs
- 20311 (Mouse); 60665 (Rat)
- Background
- LPS-Induced CXC Chemokine Ligand (LIX)
- LPS-induced CXC chemokine (LIX) is a member of the murine chemokine family. It is 132 amino acids (aa) in length with a predicted molecular weight of 14 kDa prior to cleavage of the N-terminal signal peptide (aa 1–40). LIX has no true human ortholog but is closely related to human CXCL5/ENA-78 and CXCL6/GCP-2. It is widely expressed, and is secreted by multiple cell types including fibroblasts, thymic epithelium, platelets, vascular endothelium, and hepatocytes. LIX acts as a proinflammatory chemokine and is chemotactic for neutrophils.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost. 2012 10:1616-23
Species: Mouse
Sample Type: Plasma
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