Mouse sVEGF R2/Flk-1 Quantikine ELISA Kit | msVEGF R2 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111368
R&D Systems製のMouse sVEGF R2/Flk-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年06月07日 20時55分現在]
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Flk-1, Mouse, ELISA Kit, Quantikine, 2nd Generation (96 well) <sVEGF R2, ELISA Kit> |
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[在庫・価格 ：2024年06月07日 20時55分現在]
Flk-1, Mouse, ELISA Kit, Quantikine, 2nd Generation (96 well) <sVEGF R2, ELISA Kit>
文献数： 16
- 商品コード：MVR200B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：0.16 - 10 ng/mL，感度：0.049 ng/mL，測定試料：Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：VEGF R2/KDR/Flk-1 別名：CD309 Genbank No： 3791 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 0.049 ng/mL
- Assay Range
- 0.16 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates)
- Specificity
- Natural and recombinant mouse sVEGF R2
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse soluble VEGF R2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R2 in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R2.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 24 | 24 | 24 |
| Mean | 0.63 | 1.4 | 3.83 | 0.57 | 1.28 | 3.5 |
| Standard Deviation | 0.04 | 0.07 | 0.21 | 0.06 | 0.1 | 0.21 |
| CV% | 6.3 | 5 | 5.5 | 10.5 | 7.8 | 6 |
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Recovery
The recovery of mouse sVEGF R2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 105 | 100-111 |
| Serum (n=3) | 104 | 82-120 |
| Plasma (n=4) | 108 | 89-116 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sVEGF R2 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- VEGF R2/KDR/Flk-1
- Aliases
- CD309; Flk-1; KDR
- Entrez Gene IDs
- 3791 (Human); 16542 (Mouse)
- Background
- VEGF R2
- VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoieticdevelopment. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Long-term continuous corticosterone treatment decreases VEGF receptor-2 expression in frontal cortex.
Howell KR, Kutiyanawalla A, Pillai A
PLoS ONE 2011 6:e20198
Species: Mouse
Sample Type: Serum
Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1.
Aragones J, Belin MF, Carmeliet P, Chedotal A, Chounlamountri N, Coulon C, Dassonville K, Dhondt J, D'Hooge R, Dresselaers T, Eriksson U, Gall D, Gallez B, Geudens I, Haigh JJ, Hermans K, Honnorat J, Jordan BF, Knevels E, Lambrechts D, Meissirel C, Moons L, Mutin M, Poesen K, Renaud J, Ruiz de Almodovar C, Salin PA, Schiffmann S, Storkebaum E, Stroobants S, Thomasset N, Van Geyte K, Van Hecke P, Vinckier S, Zacchigna S
J. Neurosci. 2010 30:15052-66
Species: Mouse
Sample Type: Tissue Homogenates
Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
Ishida S, Izumi-Nagai K, Nagai N, Ohgami K, Ohno S, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Invest. Ophthalmol. Vis. Sci. 2008 49:1679-85
Species: Mouse
Sample Type: Tissue Homogenates
An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
Barbieri S, Campochiaro PA, Dong A, Hackett SF, Shen J, Swaim M, Worpenberg S, Wyder L, Xie B
FASEB J. 2008 22:2775-83
Species: Mouse
Sample Type: Tissue Homogenates
(Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
Fukamizu A, Ichihara A, Ishida S, Itoh H, Nagai N, Noda K, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Am. J. Pathol. 2008 173:1911-8
Species: Mouse
Sample Type: Tissue Homogenates
Vascular endothelial growth factor-mediated decrease in plasma soluble vascular endothelial growth factor receptor-2 levels as a surrogate biomarker for tumor growth.
Alami J, Bogdanovic E, Dumont DJ, Ebos JM, Francia G, Kerbel RS, Lee CR, Mutsaers AJ, Van Slyke P, Xu P
Cancer Res. 2008 68:521-9
Species: Mouse
Sample Type: Plasma
Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
Aonuma K, Hattori Y, Jesmin S, Maeda S, Miyauchi T, Otsuki T, Shimojo N, Yamaguchi N, Zaedi S
Am. J. Physiol. Heart Circ. Physiol. 2007 293:H474-81
Species: Rat
Sample Type: Cell Culture Supernates
Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
Christensen JG, Ebos JM, Kerbel RS, Lee CR, Mutsaers AJ
Proc. Natl. Acad. Sci. U.S.A. 2007 104:17069-74
Species: Mouse
Sample Type: Plasma
Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts.
Iemitsu M, Jesmin S, Maeda S, Miyauchi T, Otsuki T
Am. J. Physiol. Heart Circ. Physiol. 2006 291:H1290-8
Species: Rat
Sample Type: Tissue Homogenates
Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor.
Ikeda E, Inoue M, Ishida S, Koto T, Kubota Y, Nagai N, Noda K, Oike Y, Shinoda H, Shinoda K, Shiomi T, Suda T, Tsubota K, Urano T
Invest. Ophthalmol. Vis. Sci. 2005 46:1078-84
Species: Mouse
Sample Type: Tissue Homogenates
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