Mouse VEGF R1/Flt-1 Quantikine ELISA Kit | mVEGF R1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111367
R&D Systems製のMouse VEGF R1/Flt-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 15時15分現在]
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Flt-1, Soluble, Mouse, ELISA Kit, Quantikine M (96 well) <sVEGF Receptor1, ELISA Kit> |
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[在庫・価格 ：2025年04月26日 15時15分現在]
Flt-1, Soluble, Mouse, ELISA Kit, Quantikine M (96 well) <sVEGF Receptor1, ELISA Kit>
文献数： 38
- 商品コード：MVR100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：125-8,000 pg/mL，感度：15.2 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：VEGFR1, Flt-1 別名：EC 2.7.10 Genbank No： 2321 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2019年12月15日号 p.3
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| 製品記事 | R&D Systems社 VEGF-VEGF R2シグナル伝達系関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL)
- Sensitivity
- 15.2 pg/mL
- Assay Range
- 125 - 8,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse sVEGF R1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse soluble VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 265 | 1,383 | 2,174 | 250 | 1,339 | 2,139 |
| Standard Deviation | 19 | 55 | 69 | 21 | 96 | 134 |
| CV% | 7.2 | 4 | 3.2 | 8.4 | 7.2 | 6.3 |
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Recovery
The recovery of mouse sVEGF R1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 106 | 96-119 |
| Cell Culture Supernates (n=6) | 96 | 82-110 |
| EDTA Plasma (n=6) | 106 | 92-120 |
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Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse sVEGF R1 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- VEGF R1/Flt-1
- Aliases
- FLT1; Flt-1
- Entrez Gene IDs
- 2321 (Human); 14254 (Mouse)
- Background
- VEGF R1, R3
- VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoieticdevelopment. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Role of reactive oxygen species during hypertension in response to chronic antiangiogenic factor (sFlt-1) excess in pregnant rats.
Arany M, Cockrell K, Fournier L, Granger JP, Lamarca B, Martin JN, Murphy S, Tam Tam KB
Am. J. Hypertens. 2011 24:110-3
Species: Rat
Sample Type: Plasma
Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
Mulik S, Sharma S, Suryawanshi A
J. Immunol. 2011 186:3653-65
Species: Mouse
Sample Type: Tissue Homogenates
Sera from preeclampsia patients elicit symptoms of human disease in mice and provide a basis for an in vitro predictive assay.
Boij R, Kalkunte S, Norris W
Am. J. Pathol. 2010 177:2387-98
Species: Mouse
Sample Type: Serum
Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization.
Aragones J, Carmeliet P, De Smet F, Debackere K, Dejana E, Dettori D, Gallez B, Jonckx B, Jordan B, Lampugnani MG, Lanahan AA, Leite de Oliveira R, Loges S, Luttun A, Maxwell P, Mazzone M, Pisacane A, Pollard P, Ratcliffe P, Ruiz de Almodovar C, Schmidt T, Simons M, Tian YM, Vinckier S, Wyns S
Cell 2009 136:839-51
Species: Mouse
Sample Type: Cell Culture Supernates
Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
Ishida S, Izumi-Nagai K, Nagai N, Ohgami K, Ohno S, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Invest. Ophthalmol. Vis. Sci. 2008 49:1679-85
Species: Mouse
Sample Type: Tissue Homogenates
Angiotensin receptor agonistic autoantibodies induce pre-eclampsia in pregnant mice.
Hicks MJ, Irani RA, Kellems RE, Mi T, Popek EJ, Ramin SM, Xia Y, Zhang H, Zhang Y, Zhou CC
Nat. Med. 2008 14:855-62
Species: Mouse
Sample Type: Plasma
An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
Barbieri S, Campochiaro PA, Dong A, Hackett SF, Shen J, Swaim M, Worpenberg S, Wyder L, Xie B
FASEB J. 2008 22:2775-83
Species: Mouse
Sample Type: Tissue Homogenates
Novel role for vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.
Bender F, Bogaert E, Carmeliet P, Claes B, Dhondt J, D'Hooge R, Eriksson U, Frank N, Heylen L, Lambrechts D, Meert T, Nuydens R, Poesen K, Raes K, Robberecht W, Sendtner M, Shibuya M, Tjwa M, Van Damme P, Van Den Bosch L, Verheyen A
J. Neurosci. 2008 28:10451-9
Species: Rat
Sample Type: Cell Lysates
(Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
Fukamizu A, Ichihara A, Ishida S, Itoh H, Nagai N, Noda K, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Am. J. Pathol. 2008 173:1911-8
Species: Mouse
Sample Type: Tissue Homogenates
Soluble vascular endothelial growth factor receptor-1 protects mice in sepsis.
Chan FT, Chen CY, Chou HC, Hsieh FJ, Hsieh WS, Hsu SM, Hsu WM, Su YN, Tsao PN, Wei SC
Crit. Care Med. 2007 35:1955-60
Species: Mouse
Sample Type: Plasma
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