Mouse VEGF R1/Flt-1 Quantikine ELISA Kit | mVEGF R1 Qkit

R&D Systems製のMouse VEGF R1/Flt-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。

The Quantikine Mouse soluble VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R1.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma

The recovery of mouse sVEGF R1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse sVEGF R1 in each matrix were diluted with Calibrator Diluent and then assayed.

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

Role of reactive oxygen species during hypertension in response to chronic antiangiogenic factor (sFlt-1) excess in pregnant rats.
Arany M, Cockrell K, Fournier L, Granger JP, Lamarca B, Martin JN, Murphy S, Tam Tam KB
Am. J. Hypertens. 2011 24:110-3
Species: Rat
Sample Type: Plasma


Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
Mulik S, Sharma S, Suryawanshi A
J. Immunol. 2011 186:3653-65
Species: Mouse
Sample Type: Tissue Homogenates


Sera from preeclampsia patients elicit symptoms of human disease in mice and provide a basis for an in vitro predictive assay.
Boij R, Kalkunte S, Norris W
Am. J. Pathol. 2010 177:2387-98
Species: Mouse
Sample Type: Serum


Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization.
Aragones J, Carmeliet P, De Smet F, Debackere K, Dejana E, Dettori D, Gallez B, Jonckx B, Jordan B, Lampugnani MG, Lanahan AA, Leite de Oliveira R, Loges S, Luttun A, Maxwell P, Mazzone M, Pisacane A, Pollard P, Ratcliffe P, Ruiz de Almodovar C, Schmidt T, Simons M, Tian YM, Vinckier S, Wyns S
Cell 2009 136:839-51
Species: Mouse
Sample Type: Cell Culture Supernates


Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
Ishida S, Izumi-Nagai K, Nagai N, Ohgami K, Ohno S, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Invest. Ophthalmol. Vis. Sci. 2008 49:1679-85
Species: Mouse
Sample Type: Tissue Homogenates


Angiotensin receptor agonistic autoantibodies induce pre-eclampsia in pregnant mice.
Hicks MJ, Irani RA, Kellems RE, Mi T, Popek EJ, Ramin SM, Xia Y, Zhang H, Zhang Y, Zhou CC
Nat. Med. 2008 14:855-62
Species: Mouse
Sample Type: Plasma


An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
Barbieri S, Campochiaro PA, Dong A, Hackett SF, Shen J, Swaim M, Worpenberg S, Wyder L, Xie B
FASEB J. 2008 22:2775-83
Species: Mouse
Sample Type: Tissue Homogenates


Novel role for vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.
Bender F, Bogaert E, Carmeliet P, Claes B, Dhondt J, D'Hooge R, Eriksson U, Frank N, Heylen L, Lambrechts D, Meert T, Nuydens R, Poesen K, Raes K, Robberecht W, Sendtner M, Shibuya M, Tjwa M, Van Damme P, Van Den Bosch L, Verheyen A
J. Neurosci. 2008 28:10451-9
Species: Rat
Sample Type: Cell Lysates


(Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
Fukamizu A, Ichihara A, Ishida S, Itoh H, Nagai N, Noda K, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Am. J. Pathol. 2008 173:1911-8
Species: Mouse
Sample Type: Tissue Homogenates


Soluble vascular endothelial growth factor receptor-1 protects mice in sepsis.
Chan FT, Chen CY, Chou HC, Hsieh FJ, Hsieh WS, Hsu SM, Hsu WM, Su YN, Tsao PN, Wei SC
Crit. Care Med. 2007 35:1955-60
Species: Mouse
Sample Type: Plasma