Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit | mThrombomodulin Qkit
掲載日情報：2016/08/18 現在Webページ番号：111359
R&D Systems製のMouse Thrombomodulin/BDCA-3を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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[在庫・価格 ：2024年05月18日 00時00分現在]
Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit
文献数： 4
- 商品コード：MTHBD0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)，測定範囲：46.90 - 3000 pg/mL，感度：19.1 pg/mL，測定波長：450 nm（補正波長：540または570 nm），アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，種交差性：Mouse，検出方法：呈色，測定時間：4.5 hours，測定因子：Thrombomodulin/BDCA-3 別名：BDCA3 Genbank No： 7056 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Mouse Thrombomodulin / BDCA-3 Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
- Sensitivity
- 19.1 pg/mL
- Assay Range
- 46.9 - 3,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant mouse Thrombomodulin.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse Thrombomodulin/BDCA-3 immunoassay is a 4.5 hour solid-phase ELISA designed to measure Thrombomodulin in mouse cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Thrombomodulin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Thrombomodulin. Results obtained using natural mouse Thrombomodulin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Thrombomodulin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 108 | 388 | 1,159 | 121 | 380 | 1,155 |
| Standard Deviation | 6.67 | 16 | 44.8 | 16 | 38.1 | 136 |
| CV% | 6.2 | 4.1 | 3.9 | 13.2 | 10 | 11.8 |
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Recovery
The recovery of mouse Thrombomodulin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 104 | 91-115 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Thrombomodulin in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. All samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- Thrombomodulin/BDCA-3
- Aliases
- BDCA-3; CD141; Fetomodulin; THBD; THRM
- Entrez Gene IDs
- 7056 (Human); 21824 (Mouse); 83580 (Rat)
- Background
- Thrombomodulin/CD141
- Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. Predominantly synthesized by vascular endothelial cells, thrombomodulin inhibits coagulation and fibrinolysis. Thrombomodulin functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Mutations in the Thrombomodulin gene can cause inherited thrombophilia. In addition, THBD gene polymorphisims are associated with human disease and thrombomodulin plays a role in thrombosis, stroke, arteriosclerosis, and cancer. For example, increased serum levels of thrombomodulin, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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