Mouse SP-D Quantikine ELISA Kit | mSP-D QKit
掲載日情報：2016/08/18 現在Webページ番号：111352
R&D Systems製のMouse SP-Dを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 17時55分現在]
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[在庫・価格 ：2025年04月26日 17時55分現在]
Mouse SP-D Quantikine ELISA Kit
文献数： 3
- 商品コード：MSFPD0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：9.43 pg/mL，測定範囲：62.5-4,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Lysate，種交差性：Mouse，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，測定時間：4.5 hours，測定因子：SP-D 別名：COLEC7collectin-7 Genbank No： 6441 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine Human / Mouse SP-D ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 9.43 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Lysates)
- Specificity
- Natural and recombinant mouse SP-D.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse SP-D immunoassay is a 4.5 hour solid-phase ELISA designed to measure SP-D in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse SP-D and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SP-D. Results obtained using natural mouse SP-D showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse SP-D.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 4 | 4 | 4 | 4 | 4 | 4 |
| Mean | 158 | 534 | 1,270 | 169 | 531 | 1,267 |
| Standard Deviation | 5.75 | 13.8 | 43 | 14 | 34.4 | 120 |
| CV% | 3.6 | 2.6 | 3.4 | 8.3 | 6.5 | 9.5 |
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Recovery
The recovery of mouse SP-D spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 99 | 92-105 |
| Tissue Lysates (n=4) | 101 | 91-113 |
| Serum (n=4) | 96 | 84-109 |
| EDTA Plasma (n=4) | 97 | 90-103 |
| Heparin Plasma (n=4) | 97 | 84-104 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SP-D in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay
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Additional Infomation
- Molecule Information
- SP-D
- Aliases
- Collectin 7; PSP-D; SFTP4; SFTPD
- Entrez Gene IDs
- 6441 (Human); 20390 (Mouse)
- Background
- SP-D
- Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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