Mouse MSP/MST1 Quantikine ELISA Kit | mMSP/MST1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111329
R&D Systems製のMouse MSP/MST1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月29日 11時55分現在]
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Mouse MSP/MST1 Quantikine ELISA Kit |
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[在庫・価格 ：2024年04月29日 11時55分現在]
Mouse MSP/MST1 Quantikine ELISA Kit
文献数： 0
- 商品コード：MMSP0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：0.019 ng/mL，測定範囲：0.16 - 10 ng/mL，測定試料：Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)，種交差性：Mouse，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：MSP/MST1 別名：D3F15S2 Genbank No： 4485 |
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| 製品記事 |
Quantikine Mouse MSP ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
- Sensitivity
- 0.019 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates, Urine)
- Specificity
- Natural and recombinant mouse MSP
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Mouse MSP/MST1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MSP in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains CHO cell-expressed recombinant mouse MSP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse MSP. Results obtained using natural mouse MSP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MSP.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.504 | 1.28 | 3.98 | 0.47 | 1.24 | 3.74 |
| Standard Deviation | 0.027 | 0.068 | 0.154 | 0.015 | 0.044 | 0.228 |
| CV% | 5.4 | 5.3 | 3.9 | 3.2 | 3.6 | 6.1 |
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Recovery
The recovery of mouse MSP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=4) | 98 | 90-108 |
| Tissue Homogenates (n=4) | 101 | 90-109 |
| Urine (n=4) | 96 | 88-104 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MSP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. 
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Additional Infomation
- Molecule Information
- MSP/MST1
- Aliases
- HGFL; MST1; SF2
- Entrez Gene IDs
- 4485 (Human); 15235 (Mouse); 24566 (Rat)
- Background
- MSP
- Macrophage-stimulating protein (MSP), also known as Hepatocyte Growth Factor-like Protein (HGFL) and Scatter Factor-2 (SF2), is a member of the HGF family of growth factors. MSP is secreted as a single chain inactive precursor (pro-MSP). In response to injury, pro-MSP is activated by proteolytic cleavage. Activation results in the creation of alpha and beta chains joined by a disulfide bond.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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