Mouse Total MMP-9 Quantikine ELISA Kit | mTotal MMP-9 QKit
掲載日情報：2016/08/18 現在Webページ番号：111327
R&D Systems製のMouse Total MMP-9を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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MMP-9, Total, Mouse, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
MMP-9, Total, Mouse, ELISA Kit, Quantikine (96well)
文献数： 37
- 商品コード：MMPT90
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.078-5 ng/mL，感度：0.014 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：Total MMP-9 別名：92 kDa gelatinase Genbank No： 4318 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine MMP-9 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.014 ng/mL
- Assay Range
- 0.078 - 5 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates)
- Specificity
- Natural and recombinant mouse MMP-9 (pro-, active, and TIMP-complexed)
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse MMP-9 (total) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-9 (pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MMP-9 and antibodies raised against the recombinant factor. Natural mouse MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-9.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.239 | 0.731 | 1.71 | 0.211 | 0.6 | 1.61 |
| Standard Deviation | 0.024 | 0.037 | 0.097 | 0.02 | 0.049 | 0.125 |
| CV% | 8.4 | 5.1 | 5.7 | 9.6 | 8.1 | 7.8 |
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Recovery
The recovery of mouse MMP-9 spiked to 3 levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 94 | 86-108 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- MMP-9
- Aliases
- CLG4B; Gelatinase B; GELB
- Entrez Gene IDs
- 4318 (Human); 17395 (Mouse); 81687 (Rat)
- Background
- Matrix Metalloproteinases (MMPs)
- The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Soluble E-cadherin: a critical oncogene modulating receptor tyrosine kinases, MAPK and PI3K/Akt/mTOR signaling.
Athar , M, Brouxhon , S M, Ghazizadeh , S, Kyrkanides , S, Ma , L, O'Banion , M K, Simon , M, Teng , X
Oncogene 2014 33:225-35
Species: Mouse
Sample Type: Cell Culture Supernates
The combination of the histone deacetylase inhibitor vorinostat and synthetic triterpenoids reduces tumorigenesis in mouse models of cancer.
Gediya , Lalji K, Liby , Karen T, Njar , Vincent , Pioli , Patricia, Risingsong , Renee, Royce , Darlene , Sporn , Michael , Tran , Kim, Williams , Charlott
Carcinogenesis 2013 34:199-210
Species: Mouse
Sample Type: cell supernates
The effects of low levels of dystrophin on mouse muscle function and pathology.
Aartsma-Rus A, Admiraal P, den Dunnen JT, Hulsker M, Messemaker T, Nadarajah VD, 't Hoen PA, van Heiningen SH, van Huizen E, van Iterson M, van Putten M
PLoS ONE 2012 7:e31937
Species: Mouse
Sample Type: Serum
The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
Risingsong R, Royce D, Tran K
Cancer Prev Res (Phila) 2012 5:726-34
Species: Mouse
Sample Type: Cell Culture Supernates
Role of c-Jun N-terminal protein kinase 1/2 (JNK1/2) in macrophage-mediated MMP-9 production in response to Moraxella catarrhalis lipooligosaccharide (LOS).
Hassan F, Ren D, Zhang W
PLoS ONE 2012 7:e37912
Species: Mouse
Sample Type: Cell Culture Supernates
A hypomorphic mutation in the Gfi1 transcriptional repressor results in a novel form of neutropenia.
Eur J Immunol 2012 42:2395-408
Species: Mouse
Sample Type: BALF
Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
Conant K, Krall C, Lim ST, Lonskaya I, Maguire-Zeiss K, Steiner J, Szklarczyk A
J. Neurochem. 2011 118:521-32
Species: Mouse
Sample Type: Cell Lysates
Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence.
Colton CA, Gordon MN, Morgan D, Ridnour LA, Taylor TL, Wilcock DM, Wink DA
J Neuroinflammation 2011 8:115
Species: Mouse
Sample Type: Tissue Homogenates
15-Epi-lipoxin A(4) inhibits the progression of endometriosis in a murine model.
Chen QH, Chen QX, Huang QS, Li T, Pu DM, Zhou WD
Fertil. Steril. 2010 93:1440-7
Species: Mouse
Sample Type: Peritoneal Fluid
Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner.
Hou YF, Liu ZB, Zhu J
Oncogene 2010 29:2996-3009
Species: Mouse
Sample Type: Serum
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