Mouse Pro-MMP-9 Quantikine ELISA Kit | mPro-MMP-9 QKit
掲載日情報：2016/08/18 現在Webページ番号：111326
R&D Systems製のMouse Pro-MMP-9を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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MMP-9, Pro-, Mouse, ELISA Kit, Quantikine (96well) <Pro-MMP-9, ELISA Kit> |
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[在庫・価格 ：2024年05月18日 00時00分現在]
MMP-9, Pro-, Mouse, ELISA Kit, Quantikine (96well) <Pro-MMP-9, ELISA Kit>
文献数： 15
- 商品コード：MMP900B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.30 - 2000 pg/mL，感度：14 pg/mL，測定試料：Cell Culture Supernates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：MMP-9 別名：92 kDa gelatinase Genbank No： 4318 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (25 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL)
- Sensitivity
- 14 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant mouse Pro-MMP-9. This kit recognizes Pro-MMP-9 when complexed with TIMP-1 but does not recognize active mouse MMP-9.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse Pro-MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure free and TIMP-1 bound mouse Pro-MMP-9 in cell culture supernates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse Pro-MMP-9 and antibodies raised against the recombinant factor. Natural mouse Pro-MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Pro-MMP-9.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 126 | 206 | 1,063 | 133 | 230 | 1,073 |
| Standard Deviation | 3.36 | 7.53 | 28.6 | 11 | 17.5 | 55.2 |
| CV% | 2.7 | 3.7 | 2.7 | 8.3 | 7.6 | 5.1 |
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Recovery
The recovery of mouse Pro-MMP-9 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=4) | 101 | 82-117 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Pro-MMP-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- MMP-9
- Aliases
- CLG4B; Gelatinase B; GELB
- Entrez Gene IDs
- 4318 (Human); 17395 (Mouse); 81687 (Rat)
- Background
- Matrix Metalloproteinases (MMPs)
- The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
TIEG1 inhibits breast cancer invasion and metastasis by inhibition of epidermal growth factor receptor (EGFR) transcription and the EGFR signaling pathway.
Chen BB, Chen JY, Gu SM, Huang M, Jin W, Li JY, Shao ZM, Wang QQ, Wu J, Yu S, Zhu H
Mol. Cell. Biol. 2012 32:50-63
Neutrophil-derived matrix metalloproteinase-9 is a potent activator of trypsinogen in acinar cells in acute pancreatitis.
Abdulla A, Awla D, Jeppsson B, Regner S, Syk I, Thorlacius H
J. Leukoc. Biol. 2012 91:711-9
IFN-beta inhibits dendritic cell migration through STAT-1-mediated transcriptional suppression of CCR7 and matrix metalloproteinase 9.
Ganea D, Kong W, Yen JH
J. Immunol. 2010 184:3478-86
Species: Mouse
Sample Type: Cell Culture Supernates
Matrix metalloproteinase (MMP)-13 regulates mammary tumor-induced osteolysis by activating MMP9 and transforming growth factor-beta signaling at the tumor-bone interface.
Futakuchi M, Nannuru KC, Varney ML
Cancer Res. 2010 70:3494-504
Species:
Sample Type: Tissue Homogenates
Targeting the chemotactic function of CD147 reduces collagen-induced arthritis.
Arora K, Bukrinsky MI, Constant SL, Damsker JM, Okwumabua I, Pushkarsky T
Immunology 2009 126:55-62
Species: Mouse
Sample Type: Cell Culture Supernates
Osteopontin deficiency protects against airway remodeling and hyperresponsiveness in chronic asthma.
Gratziou C, Panoutsakopoulou V, Petrochilou K, Roussos C, Simoes DC, Xanthou G
Am. J. Respir. Crit. Care Med. 2009 179:894-902
Species: Mouse
Sample Type: Tissue Homogenates
Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model.
Jeoung D, Kim DY, Park JW, Ro JY
Eur. J. Pharmacol. 2009 612:98-105
Species: Mouse
Sample Type: BALF
Dopamine regulates endothelial progenitor cell mobilization from mouse bone marrow in tumor vascularization.
Baral R, Basu S, Chakroborty D, Chowdhury UR, Dasgupta PS, Sarkar C
J. Clin. Invest. 2008 118:1380-9
Species: Mouse
Sample Type: Cell Culture Supernates
Retinoic acid decreases adherence of murine myeloid dendritic cells and increases production of matrix metalloproteinase-9.
Ashley SL, Davis AL, Hoag KA, Lackey DE
J. Nutr. 2008 138:1512-9
Species: Mouse
Sample Type: Cell Culture Supernates
Metalloelastase (MMP-12) induced inflammatory response in mice airways: effects of dexamethasone, rolipram and marimastat.
Berna P, Bertrand CP, Boichot E, Hitier S, Lagente V, Nenan S, Planquois JM
Eur. J. Pharmacol. 2007 559:75-81
Species: Mouse
Sample Type: Tissue Homogenates
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