Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit | mIL-1a Qkit
掲載日情報：2016/08/18 現在Webページ番号：111314
R&D Systems製のMouse IL-1 alpha/IL-1F1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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IL-1α, Mouse, ELISA Kit, Quantikine M (96 well) |
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[在庫・価格 ：2025年04月27日 00時00分現在]
IL-1α, Mouse, ELISA Kit, Quantikine M (96 well)
文献数： 47
- 商品コード：MLA00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(発注済)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：4.69-300 pg/mL，感度：2.5 pg/mL，測定試料：Serum, Cell Culture Supernatant，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-1 alpha, IL-1F1 別名：Hematopoietin-1 Genbank No： 3552 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2018年7月1日号 p.11 ニュース2019年8月15日号 p.15
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| 製品記事 | がん幹細胞関連因子測定用 Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL)
- Sensitivity
- 2.5 pg/mL
- Assay Range
- 4.69 - 300 pg/mL (Serum, Cell Culture Supernates)
- Specificity
- Natural and recombinant mouse IL-1 alpha
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Mouse IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 alpha levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-1 alpha accurately. Results obtained using natural mouse IL-1 alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 alpha.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 17 | 32 | 156 | 18 | 33 | 162 |
| Standard Deviation | 1.5 | 1 | 3.9 | 0.9 | 1.2 | 7.9 |
| CV% | 8.8 | 3.1 | 2.5 | 5 | 3.6 | 4.9 |
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Recovery
The recovery of mouse IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 94 | 88-103 |
| Cell Culture Supernates (n=5) | 102 | 92-109 |
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Linearity
To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-1 alpha/IL-1F1
- Aliases
- IL1A; IL-1F1
- Entrez Gene IDs
- 3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
- Background
- IL-1 alpha/IL-1F1
- Interleukin-1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL 4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Recent evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.
Blood 2012 120:2600-9
Species: Mouse
Sample Type: tissue lysates
Tissue-specific opposing functions of the inflammasome adaptor ASC in the regulation of epithelial skin carcinogenesis.
Bonsignore , Luca, Drexler , Stefan K, Gross , Olaf, Hermeking , Heiko, Jackstadt , Rene, Masin , Mark, Schneider , Pascal, Tardivel , Aubry, Tschopp , Jurg, Yazdi , Amir S
Proc Natl Acad Sci U S A 2012 109:18384-9
Species: Mouse
Sample Type: Tissue Homogenates
Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
Cheng F, Wang H
J. Immunol. 2011 186:3986
Species: Mouse
Sample Type: Cell Culture Supernates
Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
Bracke KR, Brusselle GG, Dupont LL, Joos GF, Lambrecht BN, Pauwels NS, Provoost S, Van Pottelberge GR, Vanden Berghe T, Vandenabeele P
Eur. Respir. J. 2011 38:1019-28
Species: Mouse
Sample Type: BALF
Sphingosine regulates the NLRP3-inflammasome and IL-1beta release from macrophages.
Brough D, Giles JA, Lopez-Castejon G, Luheshi NM
Eur. J. Immunol. 2011 42:716-25
Species: Mouse
Sample Type: Cell Culture Supernates
Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
Alm AS, Andersson R, Li K, Lu Y, Wang X, Yang D
Cytokine 2010 49:256-63
Species: Mouse
Sample Type: BALF
Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
Becker C, Chen Z, Coll RC, Dunne A, Franchi L, Harris J, Hull RL, Kahn SE, Lavelle EC, Masters SL, Mielke LA, Mills KH, Mok KH, Mullooly N, Newsholme P, Nunez G, O'Neill LA, Sharp FA, Subramanian SL, Tannahill GM, Yodoi J, Yoshihara E
Nat. Immunol. 2010 11:897-904
Species: Mouse
Sample Type: Cell Culture Supernates
A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
Arulanandam B, Berton MT, Dube P, Gao L, Lei L, Zhang J, Zhang X, Zhong G, Zhong Y
J. Immunol. 2009 183:1291-300
Species: Mouse
Sample Type: BALF
Tumor necrosis factor-alpha and interleukin-1 antagonists alleviate inflammatory skin changes associated with epidermal growth factor receptor antibody therapy in mice.
Busam KJ, Claros N, Corcoran E, Deevi D, Doody J, Mauro DJ, Plym MJ, Pytowski B, Rodeck U, Surguladze D, Tonra JR, Wang S, Witte L, Wu Y
Cancer Res. 2009 69:5643-7
Species: Mouse
Sample Type: Tissue Homogenates
Viral infection of the lungs through the eye.
Barik S, Bitko V, Musiyenko A
J. Virol. 2007 81:783-90
Species: Mouse
Sample Type: Tissue Homogenates
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