"Mouse IFN-gamma Quantikine ELISA Kit" | mIFN-g Qkit
掲載日情報：2016/08/18 現在Webページ番号：111309
R&D Systems製の"Mouse IFN-gamma"を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月08日 10時55分現在]
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IFN-γ, Mouse, ELISA Kit, Quantikine M (1 Plate) |
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[在庫・価格 ：2025年05月08日 10時55分現在]
IFN-γ, Mouse, ELISA Kit, Quantikine M (1 Plate)
文献数： 443
- 商品コード：MIF00-1
- メーカー：RSD
- 包装：1kit
- 価格：¥104,000
- 在庫：無(発注済)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：9.4-600 pg/mL，感度：2 pg/mL，測定試料：Serum, Cell Culture Supernatant，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IFN-gamma, 2 Plates 別名：IFG Genbank No： 3458 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 R&D Systems マウス／ラット用Quantikine ELISA Kit 2 plate製品の包装変更について Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL)
- Sensitivity
- 2 pg/mL
- Assay Range
- 9.4 - 600 pg/mL (Serum, Cell Culture Supernates)
- Specificity
- Natural and recombinant mouse IFN-gamma
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-gamma in cell culture supernates and mouse serum. It contains E. coli-expressed mouse IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IFN-gamma accurately. Results obtained using natural mouse IFN-gamma showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-gamma.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 44.8 | 156 | 518 | 43.3 | 144 | 509 |
| Standard Deviation | 2.2 | 5 | 14.2 | 3.6 | 14.7 | 48.6 |
| CV% | 4.9 | 3.2 | 2.7 | 8.3 | 10.2 | 9.5 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 45.1 | 143 | 528 | 42.8 | 139 | 518 |
| Standard Deviation | 2.1 | 4.4 | 11.8 | 3.6 | 13.6 | 46.5 |
| CV% | 4.7 | 3.1 | 2.2 | 8.4 | 9.8 | 9 |
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Recovery
The recovery of mouse IFN-gamma spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 97 | 91-106 |
| Cell Culture Supernates (n=7) | 105 | 98-115 |
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Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IFN-gamma in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IFN-gamma
- Aliases
- IFNG
- Entrez Gene IDs
- 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
- Background
- IFN-gamma
- Interferon-γ (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells.IFN-γ was originally characterized based on its antiviral activities. The protein also exerts anti-proliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, up-regulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments Helper T cell expansion and may be required for Helper T cell differentiation.IFN-γ exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-γ receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-γ receptor is structurally related to the IL-10 receptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Genetic vaccine for respiratory syncytial virus provides protection without disease potentiation.
Brough D, Gall J, Graham B, Johnson T, Rangel D
Mol Ther 2014 22:196-205
Species: Mouse
Sample Type: Cell Culture Supernates
Sindbis viral vectors transiently deliver tumor-associated antigens to lymph nodes and elicit diversified antitumor CD8+ T-cell immunity.
Granot , Tomer, Meruelo , Daniel, Yamanashi , Yoshihid
Mol Ther 2014 22:112-22
Species: Mouse
Sample Type: Cell Culture Supernates
Combinatorial PD-1 blockade and CD137 activation has therapeutic efficacy in murine cancer models and synergizes with cisplatin.
Dai M, Fan K, Guo Y, Hellstrom I, Hellstrom K, Hou S, Li W, Qian W, Wang H, Wei H, Zhao L
PLoS ONE 2013 8:e84927
Species: Mouse
Sample Type: cell culture supernate
Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
Aritake K, Hirai H, Nagata K, Nakamura M, Narumiya S, Otani S, Satoh T, Urade Y, Yamamoto Y, Yokozeki H
Am. J. Pathol. 2012 179:302-14
Species: Mouse
Sample Type: Tissue Homogenates
Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
Gottfries J, Melgar S, Michaelsson E
PLoS ONE 2012 7:e30005
Species: Mouse
Sample Type: Tissue Homogenates
Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Dervish S, Fulcher G, Harrison LC, Jackson CJ, Xue M
J. Biol. Chem. 2012 287:16356-64
Species: Mouse
Sample Type: Cell Culture Supernates
The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
Calderon J, Carrion J, Fresno M, Maganto-Garcia E, Punzon C, Terhorst C
PLoS Pathog. 2012 8:e1002799
Species: Mouse
Sample Type: Serum
Deletion of tristetraprolin caused spontaneous reactive granulopoiesis by a non-cell-autonomous mechanism without disturbing long-term hematopoietic stem cell quiescence.
Cheng WC, Civin CI, Heiser D, Kaplan IM, Kim MJ, Morisot S
J. Immunol. 2011 186:2826-34
Species: Mouse
Sample Type: Plasma
Serotonin activates dendritic cell function in the context of gut inflammation.
Cote F, Ghia JE, Khan WI, Li N, Mallet J, McClemens J, Suehiro Y, Wang H
Am. J. Pathol. 2011 178:662-71
Species:
Sample Type: Cell Culture Supernates
hsBAFF regulates proliferation and response in cultured CD4(+) T lymphocytes by upregulation of intracellular free Ca(2+) homeostasis.
Chen L, Chen SJ, Ma HW, Wen L, Zhang SQ, Zhang W
Cytokine 2011 53:215-22
Species: Mouse
Sample Type: Cell Culture Supernates
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