Mouse ICAM-1/CD54 Quantikine ELISA Kit | mICAM-1/CD54 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111308
R&D Systems製のMouse ICAM-1/CD54を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月31日 12時55分現在]
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ICAM-1, Soluble, Mouse, ELISA Kit, Quantikine (96 well) <CD54, ELISA Kit> |
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[在庫・価格 ：2025年05月31日 12時55分現在]
ICAM-1, Soluble, Mouse, ELISA Kit, Quantikine (96 well) <CD54, ELISA Kit>
文献数： 38
- 商品コード：MIC100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：0.31-20 ng/mL，感度：0.057 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：ICAM-1, CD54 別名：BB2 Genbank No： 3383 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | R&D Systems 社 sICAM-1/CD54関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.057 ng/mL
- Assay Range
- 0.31 - 20 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates)
- Specificity
- Natural and recombinant mouse sICAM-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse sICAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sICAM-1 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed, recombinant mouse sICAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sICAM-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.99 | 2.61 | 8.34 | 1.23 | 2.84 | 7.87 |
| Standard Deviation | 0.1 | 0.15 | 0.76 | 0.09 | 0.16 | 0.6 |
| CV% | 10.1 | 5.7 | 9.1 | 7.3 | 5.6 | 7.6 |
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Recovery
The recovery of mouse sICAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 95 | 80-119 |
| Heparin Plasma (n=7) | 104 | 85-120 |
| Cell Culture Supernates (n=4) | 104 | 98-113 |
| EDTA Plasma (n=7) | 98 | 83-113 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sICAM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- ICAM-1/CD54
- Aliases
- CD54
- Entrez Gene IDs
- 3383 (Human); 15894 (Mouse); 25464 (Rat)
- Background
- ICAM-1/CD54
- Intercellular adhesion molecule 1 (ICAM-1), also known as CD54, binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Beaumont M, de Waal Malefyt R, Emson C, Fick RB, Li Y, Louten J, Malinao MC, Mattson JD, McClanahan TK, Van Scott MR, Vega F, Wardle RL
Biomark Insights 2012 7:87-104
Species: Mouse
Sample Type: BALF
Abcg2 deficiency augments oxidative stress and cognitive deficits in Tg-SwDI transgenic mice.
J. Neurochem. 2012 233:456-469
Species: Mouse
Sample Type: Serum
Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia.
Du M, Mendez MP, Monroy YK
Respir. Res. 2011 12:12
Species: Mouse
Sample Type: BALF
Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene.
Lu J, Moochhala S, Sio SW
Am. J. Respir. Crit. Care Med. 2010 181:36-46
Species: Mouse
Sample Type: Tissue Homogenates
Early atherosclerosis and vascular inflammation in mice with diet-induced type 2 diabetes.
Bang CA, Bartels ED, Nielsen LB
Eur. J. Clin. Invest. 2009 39:190-9
Species: Mouse
Sample Type: Plasma
Role of the heparan sulfate proteoglycan syndecan-1 (CD138) in delayed-type hypersensitivity.
Echtermeyer FG, Gotte M, Kharabi Masouleh B, Kiesel L, Rops AL, Seelige R, ten Dam GB, van der Vlag J, van Kuppevelt TH, Vestweber D, Wild MK
J. Immunol. 2009 182:4985-93
Species: Mouse
Sample Type: Tissue Homogenates
Prevention of ocular inflammation in endotoxin-induced uveitis with resveratrol by inhibiting oxidative damage and nuclear factor-kappaB activation.
Ishida S, Kubota S, Kurihara T, Mochimaru H, Noda K, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Invest. Ophthalmol. Vis. Sci. 2009 50:3512-9
Species: Mouse
Sample Type: Cell Lysates
Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
Ishida S, Izumi-Nagai K, Nagai N, Ohgami K, Ohno S, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Invest. Ophthalmol. Vis. Sci. 2008 49:1679-85
Species: Mouse
Sample Type: Tissue Homogenates
Aspergillus fumigatus stimulates leukocyte adhesion molecules and cytokine production by endothelial cells in vitro and during invasive pulmonary disease.
Chiang LY, Filler SG, Gravelat FN, Patterson TF, Sheppard DC
Infect. Immun. 2008 76:3429-38
Species: Mouse
Sample Type: Tissue Homogenates
(Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
Fukamizu A, Ichihara A, Ishida S, Itoh H, Nagai N, Noda K, Oike Y, Ozawa Y, Satofuka S, Tsubota K
Am. J. Pathol. 2008 173:1911-8
Species: Mouse
Sample Type: Tissue Homogenates
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