Mouse/Rat HGF Quantikine ELISA Kit | m/rHGF QKit
掲載日情報：2016/08/18 現在Webページ番号：111307
R&D Systems製のMouse/Rat HGFを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月20日 19時15分現在]
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Mouse/Rat HGF Quantikine ELISA Kit |
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[在庫・価格 ：2024年04月20日 19時15分現在]
Mouse/Rat HGF Quantikine ELISA Kit
文献数： 19
- 商品コード：MHG00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：12.1 pg/mL，測定範囲：62.50 - 4000 pg/mL，種交差性：Mouse/Rat，測定試料：Cell Culture Supernates (50 uL), Tissue Homogenates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL)，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：HGF 別名：deafness Genbank No： 3082 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2017年10月15日号 p.7
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| 製品記事 |
Quantikine HGF ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (25 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL)
- Sensitivity
- 12.1 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Cell Culture Supernates, Platelet-poor EDTA Plasma, Tissue Homogenates)
- Specificity
- Natural and recombinant HGF. Recombinant mouse HGF single chain precursor cross-reacts 100% in this assay.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse/Rat HGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat HGF in cell culture supernates, tissue homogenates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse HGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural HGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 217 | 452 | 1,168 | 218 | 447 | 1,107 |
| Standard Deviation | 8.6 | 19.7 | 62.2 | 14.9 | 21 | 87.7 |
| CV% | 4 | 4.4 | 5.3 | 6.8 | 4.7 | 7.9 |
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Recovery
The recovery of HGF spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Mouse Cell Culture Supernates (n=4) | 98 | 85-106 |
| Mouse Tissue Homogenates (n=3) | 87 | 80-101 |
| Mouse Serum (n=4) | 96 | 84-112 |
| Rat Cell Culture Supernates (n=4) | 102 | 85-114 |
| Mouse Platelet-poor EDTA Plasma (n=4) | 104 | 101-106 |
| Rat Platelet-poor EDTA Plasma (n=4) | 108 | 100-116 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse/rat HGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- HGF
- Aliases
- Hepatopoietin A; SF
- Entrez Gene IDs
- 3082 (Human); 15234 (Mouse); 403441 (Canine)
- Background
- HGF (Hepatocyte Growth Factor)
- Hepatocyte growth factor (HGF), also known as Hepatopoietin A, is mitogenic for a variety of cell types, including endothelial and epithelial cells, melanocytes, and keratinocytes. It is identical to scatter factor, a fibroblast-derived soluble factor that promotes the dissociation of epithelial and vascular endothelial cell colonies in monolayer cultures by stimulating cell migration. HGF is secreted as an inactive single chain precursor and is converted to the heterodimeric active form by HGF activator.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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