Mouse/Rat GDF-15 Quantikine ELISA Kit | m/rGDF-15 QKit
掲載日情報：2016/08/18 現在Webページ番号：111304
R&D Systems製のMouse/Rat GDF-15を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Mouse/Rat GDF-15 Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Mouse/Rat GDF-15 Quantikine ELISA Kit
文献数： 18
- 商品コード：MGD150
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 試料に含まれる天然型または組換え体GDF-15（growth and differentiation factor-15）を，サンドイッチELISAにより比色定量するキット。感度：2.2 pg/mL，測定範囲：7.8-500 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine，種交差性：Mouse/Rat，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，測定時間：4.5 hours，測定因子：GDF-15 別名：GDF15 Genbank No： 9518 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Mouse/Rat GDF-15 Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)
- Sensitivity
- 2.2 pg/mL
- Assay Range
- 7.8 - 500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural mouse and rat GDF-15, recombinant mouse mature GDF-15, precursor GDF-15, and GDF-15/BMP-2 heterodimer. It does not detect monomeric recombinant mouse GDF-15.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse/Rat GDF-15 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat GDF-15 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant mouse GDF-15 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat GDF-15 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat GDF-15.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 25.6 | 60 | 190 | 26.7 | 63.1 | 196 |
| Standard Deviation | 0.69 | 1.42 | 5.49 | 1.62 | 2.84 | 10.7 |
| CV% | 2.7 | 2.4 | 2.9 | 6.1 | 4.5 | 5.5 |
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Recovery
The recovery of GDF-15 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Mouse Cell Culture Media (n=4) | 102 | 91-110 |
| Mouse Serum (n=4) | 91 | 82-101 |
| Mouse EDTA Plasma (n=4) | 95 | 81-109 |
| Mouse Heparin Plasma (n=4) | 95 | 87-104 |
| Mouse Urine (n=4) | 97 | 88-106 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of GDF-15 in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- GDF-15
- Aliases
- MIC-1; NAG-1; PDF; PLAB; PTGF-beta
- Entrez Gene IDs
- 9518 (Human); 23886 (Mouse); 29455 (Rat)
- Background
- Growth/Differentiation Factors (GDFs)
- Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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