Mouse Erythropoietin Quantikine ELISA Kit | mEpo QKit
掲載日情報：2016/08/18 現在Webページ番号：111293
R&D Systems製のMouse Erythropoietinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 20時35分現在]
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Mouse Erythropoietin Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月29日 20時35分現在]
Mouse Erythropoietin Quantikine ELISA Kit
文献数： 88
- 商品コード：MEP00B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 感度：46.9 pg/mL，測定範囲：47-3,000 pg/mL，種交差性：Mouse，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：Erythropoietin, EPO 別名：EP Genbank No： 2056 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | HIF（Hypoxia Inducible Factor、低酸素誘導因子）関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), Heparin Plasma (25 µL)
- Sensitivity
- 46.9 pg/mL
- Assay Range
- 47 - 3,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Tissue Homogenates)
- Specificity
- Natural and recombinant mouse Epo. Recombinant rat Epo has 100% cross-reactivity in this kit. This kit is not validated for use with rat samples, because rat sample dilution is not linear.
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Mouse Epo Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Epo in cell culture supernates, tissue homogenates, serum, and plasma. It contains antibodies raised against recombinant Epo and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Epo showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that the Quantikine Mouse Epo kit can be used to determine relative mass values for naturally occurring mouse Epo.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 196 | 258 | 704 | 180 | 239 | 700 |
| Standard Deviation | 7.7 | 7.8 | 31.2 | 17.5 | 15.3 | 17.5 |
| CV% | 3.9 | 3 | 4.4 | 9.7 | 6.4 | 2.5 |
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Recovery
The recovery of Epo spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=7) | 110 | 102-117 |
| Tissue Homogenates (n=3) | 94 | 80-111 |
| Serum (n=9) | 93 | 85-114 |
| Heparin Plasma (n=8) | 103 | 90-120 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of Epo in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- Erythropoietin
- Aliases
- EPO
- Entrez Gene IDs
- 2056 (Human); 13856 (Mouse); 24335 (Rat)
- Background
- Erythropoietin and Receptor
- Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
EPO-mediated expansion of late-stage erythroid progenitors in the bone marrow initiates recovery from sublethal radiation stress.
Bemis , Jeffrey , Kingsley , Paul D, Koniski , Anne D, McGrath , Kathleen, Palis , James, Peslak , Scott A, Wenger , Jesse
Blood 2012 120:2501-11
Species: Mouse
Sample Type: Plasma
Treatment of erythropoietin deficiency in mice with systemically administered siRNA.
Akinc , Akin, Bogorad , Roman L, Bulgakova , Elena, Chan , Amy Y, Fitzgerald , Kevin, Kaelin , William , Koteliansky , Victor, Kuchimanchi , Satya, Moslehi , Javid, Querbes , William, Wong , Jamie
Blood 2012 120:1916-22
Species: Mouse
Sample Type: Serum
Exogenous erythropoietin administration attenuates intermittent hypoxia-induced cognitive deficits in a murine model of sleep apnea.
BMC Neurosci 2012 13:77
Species: Mouse
Sample Type: Tissue Homogenates
Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design.
Bauer AP, Kosovac D, Ludwig C, Meissner S, Wagner R, Wild J
Gene Ther. 2011 18:189-98
Species: Mouse
Sample Type: Serum
A mouse model of hereditary folate malabsorption: deletion of the PCFT gene leads to systemic folate deficiency.
Cabrera RM, Salojin KV, Sun W
Blood 2011 117:4895-904
Species: Mouse
Sample Type: Serum
3'UTR-truncated Hmga2 cDNA causes MPN-like hematopoiesis by conferring a clonal growth advantage at the level of HSC in mice.
Bessler M, Ikeda K, Mason PJ
Blood 2011 117:5860-9
Species: Mouse
Sample Type: Serum
Aconitase Regulation of Erythropoiesis Correlates with a Novel Licensing Function in Erythropoietin-Induced ERK Signaling.
Bullock GC, Delehanty LL, Talbot AL
PLoS ONE 2011 6:e23850
Species: Mouse
Sample Type: Serum
General anesthetics inhibit erythropoietin induction under hypoxic conditions in the mouse brain.
Adachi T, Daijo H, Fukuda K, Hirota K, Kai S, Koyama T, Tanaka T
PLoS ONE 2011 6:e29378
Species: Mouse
Sample Type: Tissue Homogenates
A postweaning iron-adequate diet following neonatal iron deficiency affects iron homeostasis and growth in young rats.
Hegde NV, Jensen GL, Unger EL
J. Nutr. 2011 141:386-90
Species: Rat
Sample Type: Serum
Role for parasite genetic diversity in differential host responses to Trypanosoma brucei infection.
Chan CN, MacLeod A, McLellan S, Morrison LJ, Sweeney L, Tait A, Turner CM
Infect. Immun. 2010 78:1096-108
Species: Mouse
Sample Type: Plasma
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