Mouse CX3CL1/Fractalkine Quantikine ELISA Kit | mFractalkine Qkit
掲載日情報：2016/08/18 現在Webページ番号：111289
R&D Systems製のMouse CX3CL1/Fractalkineを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Fractalkine, Mouse, ELISA Kit, Quantikine (96 well) <CX3CL1, ELISA Kit> |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Fractalkine, Mouse, ELISA Kit, Quantikine (96 well) <CX3CL1, ELISA Kit>
文献数： 14
- 商品コード：MCX310
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：0.62-40 ng/mL，感度：0.32 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：CX3CL1, Fractalkine 別名：ABCD-3 Genbank No： 6376 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2018年7月1日号 p.11 ニュース2019年8月15日号 p.15
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| 製品記事 | がん幹細胞関連因子測定用 Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 0.32 ng/mL
- Assay Range
- 0.62 - 40 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates)
- Specificity
- Natural and recombinant mouse Fractalkine (chemokine and extracellular domains)
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse Fractalkine Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Fractalkine in cell culture supernates, tissue homogenates, mouse serum, and plasma. It contains E. coli-expressed recombinant mouse Fractalkine chemokine domain and antibodies raised against the recombinant protein. Results obtained for naturally occurring soluble mouse Fractalkine containing the chemokine domain and the mucin stalk domain showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Fractalkine.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 41 | 41 | 41 |
| Mean | 1.9 | 5.2 | 18.6 | 1.4 | 5.5 | 20 |
| Standard Deviation | 0.19 | 0.42 | 1.7 | 0.17 | 0.31 | 0.92 |
| CV% | 10 | 8.1 | 9.1 | 12.1 | 5.6 | 4.6 |
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Recovery
The recovery of mouse Fractalkine spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 98 | 91-107 |
| Heparin Plasma (n=6) | 95 | 81-110 |
| Cell Culture Supernates (n=5) | 101 | 94-106 |
| EDTA Plasma (n=6) | 95 | 83-109 |
| Tissue Homogenates (n=1) | 100 | 100 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse Fractalkine in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- CX3CL1/Fractalkine
- Aliases
- FKN; Fractalkine; Neurotactin
- Entrez Gene IDs
- 6376 (Human); 20312 (Mouse); 89808 (Rat)
- Background
- CX3CL1/Fractalkine
- Fractalkine, also named neurotactin, is a chemokine identified through bioinformatics. Fractalkine has a unique CX3C cysteine motif near the amino-terminus and is the one member of a fourth branch of the chemokine superfamily. The extracellular domain of human Fractalkine can be released by proteolysis, at the dibasic cleavage site proximal to the membrane to generate soluble fractalkine.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Fibroblast growth factor receptor 1 activation in mammary tumor cells promotes macrophage recruitment in a CX3CL1-dependent manner.
Beadnell , Thomas C, Griffin , Timothy , Reed , Johanna , Ryu , Yungil, Schwertfeger , Kathryn , Stone , Matthew
PLoS ONE 2012 7:e45877
Species: Mouse
Sample Type: Cell Culture Supernates
Ozone inhalation promotes CX3CR1-dependent maturation of resident lung macrophages that limit oxidative stress and inflammation.
Foster WM, Frush S, Gunn MD, Hollingsworth JW, Li Z, Liu N, Noble PW, Potts EN, Tighe RM
J. Immunol. 2011 187:4800-8
Species: Mouse
Sample Type: BALF
A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
Hogaboam CM, Kunkel SL, O'Connor EC, Trujillo G
Am. J. Pathol. 2008 172:1209-21
Species: Mouse
Sample Type: Tissue Homogenates
Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
Akimoto S, Goto T, Hayashi T, Ishida Y, Kimura A, Kondo T, Mukaida N
J. Immunol. 2008 181:4208-18
Species: Mouse
Sample Type: Peritoneal Lavage
The arthritis severity quantitative trait loci Cia4 and Cia6 regulate neutrophil migration into inflammatory sites and levels of TNF-alpha and nitric oxide.
Brenner M, Gulko PS, Laragione T, Mello A, Metz CN, Miller EJ, Sherry B, Yarlett NC
J. Immunol. 2007 178:2344-51
Species: Rat
Sample Type: Air Pouch Exudate
Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice.
Chen Q, Ren T, Tian Z, Wei H
Biochem. Biophys. Res. Commun. 2007 364:978-84
Species: Mouse
Sample Type: Cell Culture Supernates
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