Mouse C1q R1/CD93 Quantikine ELISA Kit | mC1q R1/CD93 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111279
R&D Systems製のMouse C1q R1/CD93を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 12時55分現在]
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[在庫・価格 ：2025年04月26日 12時55分現在]
Mouse C1q R1/CD93 Quantikine ELISA Kit
文献数： 1
- 商品コード：MCD930
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 種交差性：Mouse，感度：11.4 pg/mL，測定範囲：78.1-5,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Lysate，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：3.5 hours，測定因子：C1qR1, CD93 別名：AA4 Antigen Genbank No： 22918 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Quantikine Mouse C1qR1 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 11.4 pg/mL
- Assay Range
- 78.1 - 5,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse C1q R1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse C1q R1/CD93 Immunoassay is a 3.5 hour solid phase ELISA designed to measure mouse C1q R1 levels in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse C1q R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse C1q R1 accurately. Results obtained using natural mouse C1q R1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse C1q R1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 22 | 22 |
| Mean | 248 | 704 | 2,033 | 256 | 692 | 2,085 |
| Standard Deviation | 2.81 | 13.2 | 29.2 | 19.2 | 33.2 | 116 |
| CV% | 1.1 | 1.9 | 1.4 | 7.5 | 4.8 | 5.6 |
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Recovery
The recovery of mouse C1q R1 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=8) | 95 | 84-105 |
| Cell Lysates (n=4) | 110 | 102-116 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse C1q R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- C1q R1/CD93
- Aliases
- AA4 Antigen; C1q Rp; C1qRp; CD93; Collectin Receptor; Ly68
- Entrez Gene IDs
- 22918 (Human); 17064 (Mouse); 84398 (Rat)
- Background
- C1q R1
- C1q R1 is also known as C1q Rp, collectin receptor, and AA4 antigen. C1q R1 mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL, and SP-A.It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets, and cells of myeloid origin. C1q R1 has also been identified as a hematopoietic stem cell marker.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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