Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit | mBAFF/BLyS Qkit
掲載日情報：2016/08/18 現在Webページ番号：111271
R&D Systems製のMouse BAFF/BLyS/TNFSF13Bを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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BLyS, Mouse, ELISA Kit, Quantikine (96well) <BAFF, ELISA Kit><TNFSF13B, ELISA Kit> |
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[在庫・価格 ：2025年04月27日 00時00分現在]
BLyS, Mouse, ELISA Kit, Quantikine (96well) <BAFF, ELISA Kit><TNFSF13B, ELISA Kit>
文献数： 28
- 商品コード：MBLYS0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：46.9-3,000 pg/mL，感度：7.8 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：BAFF, BLyS, TNFSF13B 別名：ApoL related ligand TALL-1 Genbank No： 10673 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine BAFF ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (40 µL), Serum (17 µL), EDTA Plasma (17 µL), Heparin Plasma (17 µL)
- Sensitivity
- 7.8 pg/mL
- Assay Range
- 46.9 - 3,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse BAFF/BLyS
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Mouse BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse BAFF/BLyS and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BAFF/BLyS showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse BAFF/BLyS.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 50 | 64 | 56 |
| Mean | 125 | 288 | 1,315 | 127 | 325 | 1,262 |
| Standard Deviation | 3.6 | 7 | 30.3 | 16 | 27 | 136 |
| CV% | 2.9 | 2.4 | 2.3 | 12.6 | 8.3 | 10.8 |
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Recovery
The recovery of mouse BAFF/BLyS spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 98 | 86-115 |
| Heparin Plasma (n=6) | 94 | 85-100 |
| Cell Culture Supernates (n=6) | 103 | 90-108 |
| EDTA Plasma (n=6) | 96 | 85-103 |
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Linearity
To assess the linearity of the assay, samples containing mouse BAFF/BLyS in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- BAFF/BLyS/TNFSF13B
- Aliases
- BLyS; CD257; TALL1; THANK; TNFSF13B; ZTNF4
- Entrez Gene IDs
- 10673 (Human); 24099 (Mouse); 498666 (Rat)
- Background
- BAFF/BLyS/TNFSF13B
- BAFF (B cell activating factor), also known as BlyS (B lymphocyte stimulator) and TALL-1 (TNF- and APOL-related leukocyte expressed ligand 1) is a member of the TNF superfamily designated TNFSF13B. BAFF is expressed by monocytes and dendritic cells in the form of a membrane-anchored homotrimer which may be shed from the cell surface by Furin. BAFF binds BAFF R/TNFRSF13C, BCMA/TNFRSF17 and TACI/TNFRSF13B receptors to regulate B cell homeostasis.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Mice overexpressing BAFF develop a commensal flora-dependent, IgA-associated nephropathy.
Kujawa J, McCarthy DD, Wilson C
J. Clin. Invest. 2011 121:3991-4002
Species: Mouse
Sample Type: Serum
Secreted IgM enhances B cell receptor signaling and promotes splenic but impairs peritoneal B cell survival.
Baker N, Ehrenstein MR, Notley CA
J. Immunol. 2010 184:3386-93
Species: Mouse
Sample Type: Serum
The adaptor molecule Act1 regulates BAFF responsiveness and self-reactive B cell selection during transitional B cell maturation.
Allman D, Giltiay NV, Jorgensen TN, Li X, Lu Y
J. Immunol. 2010 185:99-109
Species: Mouse
Sample Type: Serum
Emu-BCL10 mice exhibit constitutive activation of both canonical and noncanonical NF-kappaB pathways generating marginal zone (MZ) B-cell expansion as a precursor to splenic MZ lymphoma.
Cui X, Li Z, Morris SW, Morse HC, Orihuela CJ, Qi CF, Rehg JE, Roopenian D, Sangster MY, Shin DM, Tuomanen E, Wang H, Xu W, Xue L, Zhang Q
Blood 2009 114:4158-68
Species: Mouse
Sample Type: Serum
Prevention of murine antiphospholipid syndrome by BAFF blockade.
Bethunaickan R, Davidson A, Factor SM, Huang W, Kahn P, Madaio MP, Ramanujam M, Tao H
Arthritis Rheum. 2008 58:2824-34
Species: Mouse
Sample Type: Serum
Inhibition of hedgehog signaling for the treatment of murine sclerodermatous chronic graft-versus-host disease.
Blood 0 120:2909-17
Species: Mouse
Sample Type: Serum
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